用甲苯处理的大肠杆菌细胞中DNA的复制与修复
Replication and repair of DNA in cells of Escherichia coli treated with toluene.
作者信息
Moses R E, Richardson C C
出版信息
Proc Natl Acad Sci U S A. 1970 Oct;67(2):674-81. doi: 10.1073/pnas.67.2.674.
Abstract
DNA synthesis has been studied in Escherichia coli cells made permeable to nucleotides by treatment with toluene. Replicative synthesis, as distinguished from repair synthesis, occurs at a rate comparable to that observed in vivo; it is dependent on the presence of all four deoxyribonucleoside triphosphates, but does not require exogenous DNA; and it is stimulated by ATP. Furthermore, replicative synthesis can be abolished at the restrictive temperature in DNA temperature-sensitive mutants. N-ethylmaleimide completely inhibits this type of synthesis, whereas it does not inhibit repair synthesis. Repair synthesis further differs from replicative synthesis in the following points: it does not require ATP; it persists at the restrictive temperature in DNA temperature-sensitive mutants; it can be induced by endogenous or exogenous nuclease activity; and its demonstration requires a Pol(+) strain.
摘要
已经在用甲苯处理后对核苷酸具有通透性的大肠杆菌细胞中研究了DNA合成。与修复合成不同，复制性合成的发生速率与体内观察到的速率相当；它依赖于所有四种脱氧核糖核苷三磷酸的存在，但不需要外源DNA；并且它受到ATP的刺激。此外，在DNA温度敏感突变体中，复制性合成在限制温度下可以被消除。N-乙基马来酰亚胺完全抑制这种类型的合成，而它不抑制修复合成。修复合成在以下方面进一步不同于复制性合成：它不需要ATP；它在DNA温度敏感突变体的限制温度下持续存在；它可以由内源性或外源性核酸酶活性诱导；并且其证明需要Pol(+)菌株。
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