Kowalski S, Yamane T, Fresco J R
Science. 1971 Apr 23;172(3981):385-7. doi: 10.1126/science.172.3981.385.
The nucleotide sequence of " denaturable"leucine acceptor transfer RNA (tRNA(Leu)(3)) from baker's yeast was determined on (32)P-labeled material. The molecule is 85 nucleotides long and can be folded into the "cloverleaf" model for secondary structure. The basis on which the sequence was deduced from the products of complete enzymatic digestion, prior to its unambiguous determination, is presented.
通过对用³²P标记的材料进行分析，测定了来自面包酵母的“可变性”亮氨酸受体转运核糖核酸（tRNA(Leu)(3)）的核苷酸序列。该分子长度为85个核苷酸，可折叠成二级结构的“三叶草”模型。文中介绍了在明确确定该序列之前，从完全酶促消化产物推导该序列的依据。
