Polak J M, Bloom S, Coulling I, Pearse A G
Gut. 1971 Apr;12(4):311-8. doi: 10.1136/gut.12.4.311.
Localization of the endocrine polypeptide cells responsible for ;glucagon-like immunoreactivity' in the gastrointestinal tract of the dog has been achieved with an immunofluorescent technique using antibodies raised against porcine pancreatic glucagon. The cells, for which we prefer the term ;enteroglucagon', could only be demonstrated by this technique in tissues fixed in carbodiimide. The enteroglucagon cells possess cytological, cytochemical, and ultrastructural characteristics in common with those of the pancreatic alpha(2) cell and they are equivalent in the stomach to the A cell and in the intestine to the L cell of the Wiesbaden terminology. Their distribution, predominantly in fundus and jejunum, correlates precisely with the distribution of glucagon-like immunoreactivity by radioimmunoassay and bioassay. The storage form of enteroglucagon differs in many respects from that of pancreatic glucagon although there are some close resemblances between the two forms of specific hormone-containing granule. Elucidation of the role of enteroglucagon should be assisted by the ability to demonstrate enteroglucagon cells.
利用针对猪胰高血糖素产生的抗体，通过免疫荧光技术实现了对犬胃肠道中负责“胰高血糖素样免疫反应性”的内分泌多肽细胞的定位。我们更倾向于用“肠高血糖素”这个术语来称呼这些细胞，只有在碳二亚胺固定的组织中才能通过这种技术显示出这些细胞。肠高血糖素细胞具有与胰腺α(2)细胞相同的细胞学、细胞化学和超微结构特征，在胃中它们相当于威斯巴登术语中的A细胞，在肠道中相当于L细胞。它们主要分布在胃底和空肠，这与通过放射免疫测定和生物测定法测得的胰高血糖素样免疫反应性的分布精确相关。尽管两种含特定激素的颗粒形式之间有一些相似之处，但肠高血糖素的储存形式在许多方面与胰高血糖素不同。能够显示肠高血糖素细胞应有助于阐明肠高血糖素的作用。
