Polak J M, Pearse A G, Garaud J C, Bloom S R
Gut. 1974 Sep;15(9):720-4. doi: 10.1136/gut.15.9.720.
Immunohistochemical studies using an antiserum to a pure porcine vasoactive intestinal peptide, possessing no cross reactivity against the related hormones glucagon, secretin, and gastrin-inhibitory peptide, revealed a wide distribution of vasoactive intestinal peptide cells throughout the entire length of the mammalian and avian gut. The highest numbers of cells were present in the small intestine and more particularly in the large intestine in all species investigated. Three types of endocrine cell in the mammalian gut are sufficiently widely distributed to be considered as the sites for production of vasoactive intestinal peptide. In the avian gut there are only two identifiable cell types. Sequential immunofluorescence and silver staining showed, in the bird, that the enterochromaffin (EC) cell was not responsible. This procedure could not be used in our mammalian gut samples but here serial section immunofluorescence for enteroglucagon and vasoactive intestinal peptide indicated that the two cells were not identical and that each was differently localized in the mucosa. These results leave the D cell of the Wiesbaden classification as the most likely site for the production of vasoactive intestinal peptide. The final identification must come from successful immune electron cytochemistry but this has not yet been achieved.
使用一种针对纯猪血管活性肠肽的抗血清进行的免疫组织化学研究表明，该抗血清与相关激素胰高血糖素、促胰液素和胃抑制肽无交叉反应，血管活性肠肽细胞在哺乳动物和鸟类肠道的整个长度上广泛分布。在所研究的所有物种中，小肠尤其是大肠中的细胞数量最多。哺乳动物肠道中的三种内分泌细胞分布足够广泛，可被视为产生血管活性肠肽的部位。在鸟类肠道中，只有两种可识别的细胞类型。连续免疫荧光和银染色显示，在鸟类中，肠嗜铬（EC）细胞不产生血管活性肠肽。此方法不能用于我们的哺乳动物肠道样本，但这里对肠高血糖素和血管活性肠肽进行的连续切片免疫荧光表明，这两种细胞不同，且各自在黏膜中的定位不同。这些结果使威斯巴登分类中的D细胞成为产生血管活性肠肽的最可能部位。最终鉴定必须来自成功的免疫电子细胞化学，但尚未实现。
