Ward C B, Glaser D A
Proc Natl Acad Sci U S A. 1971 May;68(5):1061-4. doi: 10.1073/pnas.68.5.1061.
The rates of cell growth and of DNA synthesis have been measured in synchronized cultures of Escherichia coli B/r growing with three different doubling times, from 27 to 80 min. Cell volume was found to increase linearly over the cell cycle, the rate of increase doubling at a precise cell age for each growth rate. The time at which the rate of cell growth doubled was also found to be the time at which new rounds of DNA synthesis were initiated. However, it was found that these two events could be separated in time by inhibiting DNA synthesis for a short period by the addition of nalidixic acid. It is concluded that neither event is the direct trigger for the other.
在大肠杆菌B/r的同步培养物中，已测定了以三种不同倍增时间（从27分钟到80分钟）生长时的细胞生长速率和DNA合成速率。发现细胞体积在细胞周期中呈线性增加，对于每种生长速率，增加速率在精确的细胞年龄时加倍。还发现细胞生长速率加倍的时间也是新一轮DNA合成开始的时间。然而，发现通过添加萘啶酸短时间抑制DNA合成，这两个事件在时间上可以分开。得出的结论是，这两个事件都不是另一个事件的直接触发因素。
