核糖体颗粒的物理化学及光散射研究
Physicochemical and light scattering studies on ribosome particles.
作者信息
Scafati A R, Stornaiuolo M R, Novaro P
出版信息
Biophys J. 1971 Apr;11(4):370-84. doi: 10.1016/S0006-3495(71)86221-5.
Abstract
The light scattering technique has been used to measure the molecular weight of Escherichia coli ribosomes. The 30S, 50S, and 70S components have been isolated and purified. The refractive index increment dn/dc was found to have the same value, (0.20 +/-0.01) cm(3)/g, for the three species. The molecular weights are (1.0 +/-0.1).10(6), (1.7 +/-0.1).10(6), and (2.9 +/-0.3).10(6) daltons respectively. Some information about the dimensions in solution (radius of gyration) and the interaction constant (second virial coefficient) have been obtained, and their significance is discussed.
摘要
光散射技术已被用于测量大肠杆菌核糖体的分子量。30S、50S和70S组分已被分离和纯化。发现这三种物质的折射率增量dn/dc具有相同的值，即(0.20±0.01) cm³/g。分子量分别为(1.0±0.1)×10⁶、(1.7±0.1)×10⁶和(2.9±0.3)×10⁶道尔顿。已获得了一些关于溶液中尺寸（回转半径）和相互作用常数（第二维里系数）的信息，并对其意义进行了讨论。
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