大肠杆菌50S核糖体亚基中的不对称性。
Asymmetry in the 50S ribosomal subunit of Escherichia coli.
作者信息
Moore P B, Engelman D M, Schoenborn B P
出版信息
Proc Natl Acad Sci U S A. 1974 Jan;71(1):172-6. doi: 10.1073/pnas.71.1.172.
Abstract
A substantial separation of the centers of mass of the protein and RNA portions of the 50S ribosomal subunit has been achieved using neutron scattering. This separation reveals that the subunit has a protein-rich side, a finding which is inconsistent with many models proposed for the structure. By analyzing the variation of the radius of gyration of the subunit under conditions in which the contributions of the protein and RNA were separately enhanced by deuteration and comparing these radii to the undeuterated subunit radius, we have obtained the following values. The radius of gyration of the whole particle is 78.0 +/- 0.95 A, that of the RNA moiety is 72.5 +/- 1.5 A, and that of the protein is 73.4 +/- 2.0 A. These data give a separation of the centers of mass of the RNA and protein distributions of 57.7 +/- 10 A.
摘要
利用中子散射已实现了50S核糖体亚基蛋白质和RNA部分质心的显著分离。这种分离表明该亚基有一个富含蛋白质的面，这一发现与许多提出的结构模型不一致。通过分析在氘代分别增强蛋白质和RNA贡献的条件下亚基回转半径的变化，并将这些半径与未氘代的亚基半径进行比较，我们得到了以下数值。整个颗粒的回转半径为78.0±0.95埃，RNA部分的回转半径为72.5±1.5埃，蛋白质的回转半径为73.4±2.0埃。这些数据得出RNA和蛋白质分布质心的分离为57.7±10埃。
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