Bitter G A, Roeder R G
Nucleic Acids Res. 1979 Sep 25;7(2):433-52. doi: 10.1093/nar/7.2.433.
The transcription of chromatin from adenovrius 2 transformed rat cells by murine plasmacytoma RNA polymerases I, II and III has been studied. Both the total RNA synthesis and transcription of the integrated adenovirus 2 genes by RNA polymerase II represent de novo DNA transcription as assessed by their sensitivity to actinomycin D. It is shown that each RNA polymerase class has characteristic ionic strength activation profiles and metal ion requirements. RNA polymerase II transcribes the integrated adenovirus 2 genes in chromatin at a frequency 25- to 50-fold higher than their sequences are represented in the genome. In contrast, no detectable viral RNA is synthesized when deproteinized DNA is transcribed. In the presence of Mn2+, all three RNA polymerases (I, II and III) transcribe the integrated viral genes at approximately the same relative frequencey. However, the Mg2+ as divalent cation, the proportion of the total RNA which represents viral gene transcripts is increased 3- to 4-fold with RNA polymerase II, while it remains unchanged for RNA polymerases I or III.
对鼠浆细胞瘤RNA聚合酶I、II和III转录腺病毒2转化大鼠细胞染色质的情况进行了研究。通过放线菌素D敏感性评估，RNA聚合酶II对整合的腺病毒2基因的总RNA合成和转录均代表从头DNA转录。结果表明，每种RNA聚合酶类别都有其独特的离子强度激活曲线和金属离子需求。RNA聚合酶II转录染色质中整合的腺病毒2基因的频率比其在基因组中的序列代表频率高25至50倍。相比之下，转录脱蛋白DNA时未检测到病毒RNA合成。在存在Mn2+的情况下，所有三种RNA聚合酶（I、II和III）以大致相同的相对频率转录整合的病毒基因。然而，以Mg2+作为二价阳离子时，代表病毒基因转录本的总RNA比例，RNA聚合酶II增加了3至4倍，而RNA聚合酶I或III则保持不变。
