体外从鸭网织红细胞染色质合成珠蛋白核糖核酸。
Synthesis of globin ribonucleic acid from duck-reticulocyte chromatin in vitro.
作者信息
Axel R, Cedar H, Felsenfeld G
出版信息
Proc Natl Acad Sci U S A. 1973 Jul;70(7):2029-32. doi: 10.1073/pnas.70.7.2029.
Abstract
The proteins of chromatin serve to restrict the transcription of DNA. The relevance of these findings to the control of gene expression is contingent upon the demonstration that this restriction is specific and mirrors the patterns of RNA synthesis observed in vivo. In this study we demonstrate by RNA-DNA hybridization that the vast majority of the chromatin-directed RNA is synthesized from the unique regions of the reticulocyte genome. Furthermore, by use of the DNA complement of globin mRNA as a probe in annealing reactions, de novo synthesis of globin RNA was detected in RNA transcripts from duck reticulocyte chromatin. No globin sequences were detected in similar preparations of RNA in vitro either from liver chromatin or from DNA freed of protein. These results show that the proteins of chromatin serve to restrict transcription in a very specific manner and provide convincing evidence for the existence of transcriptional control factors in eukaryotes.
摘要
染色质蛋白质的作用是限制DNA的转录。这些发现与基因表达调控的相关性取决于能否证明这种限制是特异性的，并且反映了体内观察到的RNA合成模式。在本研究中，我们通过RNA-DNA杂交证明，绝大多数由染色质指导合成的RNA是从网织红细胞基因组的独特区域合成的。此外，在退火反应中，使用珠蛋白mRNA的DNA互补物作为探针，在鸭网织红细胞染色质的RNA转录物中检测到了珠蛋白RNA的从头合成。在来自肝脏染色质或去除蛋白质的DNA的类似体外RNA制备物中未检测到珠蛋白序列。这些结果表明，染色质蛋白质以非常特异的方式限制转录，并为真核生物中存在转录控制因子提供了令人信服的证据。
相似文献
1
Synthesis of globin ribonucleic acid from duck-reticulocyte chromatin in vitro.体外从鸭网织红细胞染色质合成珠蛋白核糖核酸。
Proc Natl Acad Sci U S A. 1973 Jul;70(7):2029-32. doi: 10.1073/pnas.70.7.2029.
2
Tissue-specific transcription of the globin gene in isolated chromatin.分离染色质中珠蛋白基因的组织特异性转录。
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3440-2. doi: 10.1073/pnas.70.12.3440.
3
Nonhistone proteins control gene expression in reconstituted chromatin.非组蛋白在重构染色质中控制基因表达。
Proc Natl Acad Sci U S A. 1974 Dec;71(12):5057-61. doi: 10.1073/pnas.71.12.5057.
4
Analysis of in vitro transcription of duck reticulocyte chromatin using mercury-substituted ribonucleoside triphosphates.使用汞取代的三磷酸核糖核苷对鸭网织红细胞染色质进行体外转录分析。
Biochemistry. 1977 Nov 15;16(23):5135-45. doi: 10.1021/bi00642a029.
5
Cell-free transcription of mammalian chromatin: transcription of globin messenger RNA sequences from bone-marrow chromatin with mammalian RNA polymerase.哺乳动物染色质的无细胞转录：用哺乳动物RNA聚合酶从骨髓染色质转录珠蛋白信使RNA序列
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1219-23. doi: 10.1073/pnas.71.4.1219.
6
The structure of the globin genes in chromatin.染色质中珠蛋白基因的结构。
Biochemistry. 1975 Jun 3;14(11):2489-95. doi: 10.1021/bi00682a031.
7
In vitro transcription of chromatin in the presence of a mercurated nucleotide.在含有汞化核苷酸的情况下对染色质进行体外转录。
Proc Natl Acad Sci U S A. 1976 May;73(5):1564-7. doi: 10.1073/pnas.73.5.1564.
8
Messenger RNA in eukaryotic cells: the life history of duck globin messenger RNA.真核细胞中的信使核糖核酸：鸭珠蛋白信使核糖核酸的生命历程
Acta Endocrinol Suppl (Copenh). 1973;180:95-129. doi: 10.1530/acta.0.074s095.
9
Transcription of globin genes in reticulocyte chromatin.
FEBS Lett. 1979 Sep 1;105(1):131-6. doi: 10.1016/0014-5793(79)80902-3.
10
Is there specific transcription from isolated chromatin?从分离的染色质中是否存在特异性转录？
Nucleic Acids Res. 1978 Apr;5(4):1237-52. doi: 10.1093/nar/5.4.1237.
引用本文的文献
1
Towards a comprehensive catalogue of validated and target-linked human enhancers.迈向一个全面的已验证和与靶标相关的人类增强子目录。
Nat Rev Genet. 2020 May;21(5):292-310. doi: 10.1038/s41576-019-0209-0. Epub 2020 Jan 27.
2
Transcriptional enhancers: from properties to genome-wide predictions.转录增强子：从特性到全基因组预测。
Nat Rev Genet. 2014 Apr;15(4):272-86. doi: 10.1038/nrg3682. Epub 2014 Mar 11.
3
Epigenetics of haematopoietic cell development.造血细胞发育的表观遗传学。
Nat Rev Immunol. 2011 Jun 10;11(7):478-88. doi: 10.1038/nri2991.
4
Reconstitution of a deoxyribonuclease I-sensitive structure on active genes.活性基因上脱氧核糖核酸酶I敏感结构的重建。
Proc Natl Acad Sci U S A. 1980 Apr;77(4):1787-90. doi: 10.1073/pnas.77.4.1787.
5
Accurate initiation of human epsilon-globin RNA synthesis by Escherichia coli RNA polymerase in isolated nuclei of K562 erythroleukemia cells.大肠杆菌RNA聚合酶在K562红白血病细胞分离细胞核中准确起始人ε-珠蛋白RNA合成。
Proc Natl Acad Sci U S A. 1984 Jul;81(13):4051-4. doi: 10.1073/pnas.81.13.4051.
6
Toluidine blue binding capacity of heterochromatin and euchromatin of Triatoma infestans Klug.克氏锥蝽异染色质和常染色质的甲苯胺蓝结合能力
Histochemistry. 1980;69(2):181-7. doi: 10.1007/BF00533135.
7
Feulgen-DNA absorption curves of polytene chromosome regions of Rhynchosciara americana.美洲大蠊多线染色体区域的孚尔根-DNA吸收曲线。
Histochem J. 1980 Sep;12(5):499-511. doi: 10.1007/BF01011924.
8
Features of in vitro puffing and RNA synthesis in polytene chromosomes of Chironomus.摇蚊多线染色体的体外膨胀及RNA合成的特征
Chromosoma. 1980;78(1):33-78. doi: 10.1007/BF00291908.
9
Changes in the number of binding sites for ribonucleic acid polymerase in chromatin of WI-38 fibroblasts stimulated to proliferate.受刺激增殖的WI-38成纤维细胞染色质中核糖核酸聚合酶结合位点数量的变化。
Biochem J. 1974 Jul;141(1):27-34. doi: 10.1042/bj1410027.
10
[Structure and function of chromosomes].[染色体的结构与功能]
Naturwissenschaften. 1974 Jul;61(7):317-24. doi: 10.1007/BF00599562.
本文引用的文献
1
The Biology of Isolated Chromatin: Chromosomes, biologically active in the test tube, provide a powerful tool for the study of gene action.孤立染色质的生物学：在试管中具有生物活性的染色体为研究基因作用提供了一个有力的工具。
Science. 1968 Jan 5;159(3810):47-56. doi: 10.1126/science.159.3810.47.
2
Giant-size rapidly labeled nuclear ribonucleic acid and cytoplasmic messenger ribonucleic acid in immature duck erythrocytes.未成熟鸭红细胞中的巨大快速标记核糖核酸和细胞质信使核糖核酸
J Mol Biol. 1966 Sep;20(1):145-82. doi: 10.1016/0022-2836(66)90123-9.
3
Organ-specific restriction of transcription in mammalian chromatin.哺乳动物染色质中转录的器官特异性限制。
J Mol Biol. 1968 Jul 14;34(2):305-16. doi: 10.1016/0022-2836(68)90255-6.
4
Circular dichroism of calf liver nucleohistone.小牛肝核组蛋白的圆二色性
Biochemistry. 1970 Aug 4;9(16):3103-9. doi: 10.1021/bi00818a001.
5
Sequence-specific interaction of DNA and chromosomal protein.DNA与染色体蛋白的序列特异性相互作用。
J Mol Biol. 1969 Jan;39(2):351-64. doi: 10.1016/0022-2836(69)90322-2.
6
Fractionation of chromatin by thermal chromatography.通过热色谱法对染色质进行分级分离。
Biochemistry. 1972 Mar 14;11(6):998-1003. doi: 10.1021/bi00756a008.
7
DNA-RNA hybridization in vast DNA excess.在大量DNA过量情况下的DNA-RNA杂交
Nat New Biol. 1971 May 5;231(18):8-12.
8
Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms.DNA中的重复序列。数以十万计的DNA序列拷贝已被纳入高等生物的基因组中。
Science. 1968 Aug 9;161(3841):529-40. doi: 10.1126/science.161.3841.529.
9
Isolation of eukaryotic messenger RNA on cellulose and its translation in vitro.真核生物信使核糖核酸在纤维素上的分离及其体外翻译
Biochem Biophys Res Commun. 1972 Nov 1;49(3):680-9. doi: 10.1016/0006-291x(72)90465-2.
10
Evidence for translation of viral-specific RNA in cells of a mouse mammary carcinoma.小鼠乳腺癌细胞中病毒特异性RNA翻译的证据。
Proc Natl Acad Sci U S A. 1972 Mar;69(3):535-8. doi: 10.1073/pnas.69.3.535.
Zotero 插件