Sklan D
Poult Sci. 1979 Jul;58(4):885-9. doi: 10.3382/ps.0580885.
Digestion and absorption of lipids were determined in 3-week-old chicks fed diets containing triglycerides, free fatty acids, or free fatty acids with added glycerol. The 91YCl3 was present as a non-absorbed reference substance. Overall absorption of total fatty acids was highest in triglyceride-fed chicks and lowest in chicks receiving fatty acids. Net secretion of endogenous fatty acids from biliary phospholipids was observed in the duodenum of triglyceride fed chicks, whereas in the other treatments net absorption was observed at this site. Fractionation of the intestinal lipids by thin layer chromatography showed that triglycerides were almost completely hydrolyzed in the duodenum. Monoglycerides were present at high levels in the duodenum of triglyceride fed chicks and at lower levels in chicks receiving free fatty acids and glycerol and at trace levels in free fatty acid-fed chicks. Total micellar fatty acids were lowest in the duodenum of free fatty acid-fed chicks. In vitro incubation of pancreatic homogenate with oleic acid, glycerol, phosphatidyl choline, and taurocholate revealed synthesis of monoglycerides. The poorer fat absorption observed on feeding acidulated soapstock instead of triglycerides is partially explained by less efficient micellarization when free fatty acids are fed.
在3周龄雏鸡中测定了脂质的消化和吸收情况，这些雏鸡被喂食含有甘油三酯、游离脂肪酸或添加甘油的游离脂肪酸的日粮。91YCl3作为一种不被吸收的参考物质存在。总脂肪酸的总体吸收在喂食甘油三酯的雏鸡中最高，在接受脂肪酸的雏鸡中最低。在喂食甘油三酯的雏鸡十二指肠中观察到胆汁磷脂中内源性脂肪酸的净分泌，而在其他处理中，在该部位观察到净吸收。通过薄层色谱法对肠道脂质进行分级分离表明，甘油三酯在十二指肠中几乎完全水解。甘油单酯在喂食甘油三酯的雏鸡十二指肠中含量很高，在接受游离脂肪酸和甘油的雏鸡中含量较低，在喂食游离脂肪酸的雏鸡中含量微量。游离脂肪酸喂养的雏鸡十二指肠中总胶粒脂肪酸含量最低。胰匀浆与油酸、甘油、磷脂酰胆碱和牛磺胆酸盐的体外孵育显示了甘油单酯的合成。喂食酸化皂脚而非甘油三酯时观察到的较差脂肪吸收部分原因是喂食游离脂肪酸时胶粒化效率较低。
