Deakin J F, File S E, Hyde J R, Macleod N K
Pharmacol Biochem Behav. 1979 May;10(5):687-94. doi: 10.1016/0091-3057(79)90321-6.
Microinjections of 5,7 -dihydroxytryptamine into both the dorsal and median raphe nuclei resulted in 70-85% depletions in striatal and hippocampal 5-HT concentrations but did not affect habituation of orienting in the lick-distraction test, habituation of activity in an open-field or habituation of exploration in the holeboard test. Lesioned animals were hypoactive in the latter two tests and defaecated more than control rats in the open-field suggesting an increase in emotionality or fear. Rats with selective 5,7 -dihydroxtryptamine lesions of either the dorsal or the median raphe nucleus also showed no impairment of habituation of orienting or exploration. However, median raphe lesioned animals were hyperactive at some stages of the holeboard test. In contrast to previous studies, the results suggest intact ascending 5-HT pathways are not necessary for behavioural habituation of orienting, activity or exploration. Rather, 5-HT neurones may be involved in modulation of activity or responsiveness to aversive environments.
向背侧和中缝核内微量注射5,7-二羟基色胺，可使纹状体和海马中的5-羟色胺浓度降低70%-85%，但不影响舔舐分散试验中的定向习惯化、旷场试验中的活动习惯化或洞板试验中的探索习惯化。在旷场试验中，损伤动物在后两项试验中活动不足，且排便次数多于对照大鼠，提示情绪或恐惧增加。选择性损伤背侧或中缝核的5,7-二羟基色胺的大鼠在定向或探索习惯化方面也未表现出受损。然而，中缝核损伤的动物在洞板试验的某些阶段活动过度。与先前的研究相反，结果表明完整的5-羟色胺上行通路对于定向、活动或探索的行为习惯化并非必需。相反，5-羟色胺能神经元可能参与对厌恶环境的活动或反应性的调节。
