大肠杆菌己糖-6-磷酸转运系统的外源诱导动力学
Kinetics of exogenous induction of the hexose-6-phosphate transport system of Escherichia coli.
作者信息
Winkler H H
出版信息
J Bacteriol. 1971 Jul;107(1):74-8. doi: 10.1128/jb.107.1.74-78.1971.
Abstract
The kinetics of the exogenous induction of the hexose-phosphate transport system by glucose-6-phosphate (G6P) was investigated. The induction of this system by extracellular but not intracellular G6P was confirmed. The differential rate of synthesis was linear, a function of the extracellular concentration of G6P and independent of the previous induction history of the culture. Neither maintenance nor autocatalysis, phenomena described in the induction of the lac operon, were observed in the exogenous induction of hexose-phosphate transport. Fructose-6-phosphate, a potent competitive inhibitor of G6P influx, had no effect on the induction of the system by G6P, indicating that the transport of inducer was not involved in the induction process.
摘要
研究了葡萄糖-6-磷酸（G6P）对外源诱导己糖磷酸转运系统的动力学。证实了该系统由细胞外而非细胞内的G6P诱导。合成的差异速率呈线性，是细胞外G6P浓度的函数，且与培养物先前的诱导历史无关。在己糖磷酸转运的外源诱导中未观察到乳糖操纵子诱导中描述的维持或自催化现象。6-磷酸果糖是G6P流入的有效竞争性抑制剂，对G6P诱导该系统没有影响，表明诱导剂的转运不参与诱导过程。
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