Tandler C J, Libanati C M, Sanchis C A
J Cell Biol. 1970 May;45(2):355-66. doi: 10.1083/jcb.45.2.355.
Potassium pyroantimonate, when used as fixative (saturated or half-saturated, without addition of any conventional fixative) has been demonstrated to produce intracellular precipitates of the insoluble salts of calcium, magnesium, and sodium and to preserve the general cell morphology. In both animal and plant tissues, the electron-opaque antimonate precipitates were found deposited in the nucleus-as well as within the nucleolus-and in the cytoplasm, largely at the site of the ribonucleoprotein particles; the condensed chromatin appeared relatively free of precipitates. The inorganic cations are probably in a loosely bound state since they are not retained by conventional fixatives. The implications of this inorganic cation distribution in the intact cell are discussed in connection with their anionic counterparts, i.e., complexing of cations by fixed anionic charges and the coexistence of a large pool of inorganic orthophosphate anions in the nucleus and nucleolus.
焦锑酸钾用作固定剂（饱和或半饱和，不添加任何传统固定剂）时，已被证明能产生钙、镁和钠的不溶性盐的细胞内沉淀物，并能保持细胞的总体形态。在动物和植物组织中，电子不透明的锑酸盐沉淀物都沉积在细胞核以及核仁内，还沉积在细胞质中，主要是在核糖核蛋白颗粒的部位；浓缩的染色质似乎相对没有沉淀物。无机阳离子可能处于松散结合状态，因为它们不会被传统固定剂保留。结合它们的阴离子对应物，即固定阴离子电荷对阳离子的络合以及细胞核和核仁中大量无机正磷酸盐阴离子的共存，讨论了完整细胞中这种无机阳离子分布的意义。
