Smilowitz H, Lodish H, Robbins P W
J Virol. 1971 Jun;7(6):776-82. doi: 10.1128/JVI.7.6.776-782.1971.
A method which allows one to follow the synthesis of the major f1 coat protein in normal, unirradiated f1-infected cells is reported. The N-terminal tryptic peptide of this protein, labeled with (14)C-lysine, has a negative charge at pH 4.5 and is readily separated from the contaminating peptides of host cell proteins. This technique was used to study several aspects of the synthesis of the major f1 coat protein in infected cells.
报道了一种能让人追踪正常、未受辐射的f1感染细胞中主要f1衣壳蛋白合成过程的方法。该蛋白的N端胰蛋白酶肽段用（14）C-赖氨酸标记，在pH 4.5时带负电荷，很容易与宿主细胞蛋白的污染肽段分离。这项技术被用于研究感染细胞中主要f1衣壳蛋白合成的几个方面。
