用于检测和计数果胶分解菌的改良固体培养基。
Improved solid medium for the detection and enumeration of pectolytic bacteria.
作者信息
Hankin L, Zucker M, Sands D C
出版信息
Appl Microbiol. 1971 Aug;22(2):205-9. doi: 10.1128/am.22.2.205-209.1971.
Abstract
An improved solid agar medium (MP medium) has been developed which allows detection of pectolytic activity in bacteria. Organisms tested exhibited a variety of regulatory controls governing pectate lyase synthesis. The medium contains mineral salts, pectin, and yeast extract. After growth of the organisms, the agar plate is flooded with a polysaccharide precipitant, and pectolytic activity is shown by clear zones around active colonies. High concentrations of phosphate are shown to be necessary for pectic enzyme formation on solid media. The medium has successfully been used to detect pectolytic organisms in soil, forest litter, and rotting vegetable samples.
摘要
已开发出一种改良的固体琼脂培养基（MP培养基），可用于检测细菌中的果胶分解活性。所测试的微生物表现出多种控制果胶酸裂解酶合成的调节机制。该培养基含有矿物盐、果胶和酵母提取物。微生物生长后，用一种多糖沉淀剂淹没琼脂平板，活性菌落周围的透明区域表明存在果胶分解活性。结果表明，在固体培养基上形成果胶酶需要高浓度的磷酸盐。该培养基已成功用于检测土壤、森林凋落物和腐烂蔬菜样品中的果胶分解微生物。
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