用于假单胞菌分离和计数的新培养基。
New medium for the isolation and enumeration of pseudomonads.
作者信息
Solberg M, O'Leary V S, Riha W E
出版信息
Appl Microbiol. 1972 Oct;24(4):544-50. doi: 10.1128/am.24.4.544-550.1972.
Abstract
A new medium containing 200 ppb of 2-hydroxy-2',4,4'-trichlorodiphenyloxide (CH3565) and 10 ppm of cetyl-trimethyl-ammonium bromide (Cetrimide) in tryptic soy agar was developed and tested with 19 pure cultures of Pseudomonas, 20 microorganisms of other genera, commercially prepared ground beef, and laboratory-prepared inoculated ground beef. The new medium, CETCH agar, was compared with an antibiotic-containing medium. CETCH agar provided greater pseudomonad recoveries, a shorter incubation period prior to plate counting, and greater ease of preparation.
摘要
一种新的培养基被研发出来，其在胰蛋白胨大豆琼脂中含有200 ppb的2-羟基-2',4,4'-三氯二苯醚（CH3565）和10 ppm的十六烷基三甲基溴化铵（西曲溴铵），并使用19种假单胞菌纯培养物、20种其他属的微生物、市售绞碎牛肉以及实验室制备的接种绞碎牛肉进行了测试。这种新的培养基，即CETCH琼脂，与一种含抗生素的培养基进行了比较。CETCH琼脂能实现更高的假单胞菌回收率，在平板计数前的培养期更短，且制备更简便。
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