Whitman P A, Marshall R T
Appl Microbiol. 1971 Aug;22(2):220-3. doi: 10.1128/am.22.2.220-223.1971.
Thirty-eight bacteriophage-host systems were isolated from 22 of 45 refrigerated food products examined under psychrophilic conditions. Isolates were obtained from ground beef, pork sausage, chicken, raw skim milk, and oysters, whereas no isolations were made from liquid egg whites and processed meat products. Thirty of the 38 psychrophilic bacterial hosts were gram-negative rods, and 27 of these were classified within the genus Pseudomonas; three were members of the family Enterobacteriaceae. The remaining eight were gram-positive cocci, which were tentatively classified as Leuconostoc. Plate counts of psychrophilic bacteria were greater than 2.2 x 10(5)/ml (g) in all but one sample which contained phage, whereas phage titers ranged from less than 100 to 6.3 x 10(6) plaque-forming units/ml (g). Phage isolates showed limited host ranges usually attacking only those hosts upon which they were isolated. Of eight phages tested against 13 cultures of known identity, one showed lytic action, and this was against strains of P. fragi.
在嗜冷条件下对45种冷藏食品中的22种进行检测，从中分离出38个噬菌体-宿主系统。分离物取自碎牛肉、猪肉香肠、鸡肉、生脱脂牛奶和牡蛎，而未从蛋清液和加工肉类产品中分离出噬菌体。38个嗜冷细菌宿主中有30个是革兰氏阴性杆菌，其中27个属于假单胞菌属；3个是肠杆菌科成员。其余8个是革兰氏阳性球菌，暂定为明串珠菌属。除一个含噬菌体的样本外，所有样本中嗜冷细菌的平板计数均大于2.2×10⁵/ml（g），而噬菌体效价范围从小于100到6.3×10⁶噬菌斑形成单位/ml（g）。噬菌体分离物显示出有限的宿主范围，通常仅攻击分离出它们的宿主。针对13种已知身份的培养物测试的8种噬菌体中，有一种表现出裂解作用，且是针对弗氏假单胞菌菌株。
