Olsen R H, Metcalf E S, Todd J K
J Virol. 1968 Apr;2(4):357-64. doi: 10.1128/JVI.2.4.357-364.1968.
Ten Pseudomonas phage were isolated by sewage enrichment. Five psychrophilic and five mesophilic phage were selected for a description of some of their biological properties. In addition to growth on psychrophilic hosts, the psychrophilic phage studied were also able to grow on a mesophilic host within its growth temperature range. Latent periods for psychrophilic phage at 3.5 C were 6 to 12 hr and at 25 C were 30 to 60 min. Mesophilic phage had a latent period of 85 to 190 min at 25 C and 35 to 85 min at 37 C. Psychrophilic phage were significantly more heat-sensitive than the mesophilic phage. Of all the parameters studied, only thermal sensitivity correlated with growth at 3.5 C. Phage used in this study had a deoxyribonucleic acid base composition ranging from 39.6 to 68.2% guanine plus cytosine, deduced from melting temperature measurements.
通过污水富集分离出10株假单胞菌噬菌体。选择了5株嗜冷噬菌体和5株嗜温噬菌体来描述它们的一些生物学特性。除了能在嗜冷宿主上生长外，所研究的嗜冷噬菌体在其生长温度范围内也能在嗜温宿主上生长。嗜冷噬菌体在3.5℃时的潜伏期为6至12小时，在25℃时为30至60分钟。嗜温噬菌体在25℃时的潜伏期为85至190分钟，在37℃时为35至85分钟。嗜冷噬菌体比嗜温噬菌体对热更敏感。在所有研究的参数中，只有热敏感性与在3.5℃下的生长相关。本研究中使用的噬菌体的脱氧核糖核酸碱基组成，根据解链温度测量推断，鸟嘌呤加胞嘧啶的含量在39.6%至68.2%之间。
