Wegesa P, Sulzer A J, Van Orden A
Immunology. 1971 Nov;21(5):805-8.
A stable cuticular slide antigen was prepared for use in the indirect fluorescent antibody (IFA) test for trichinosis. Cuticles of larvae, prepared by pepsin digestion, were embedded in Tissue-Tek OCT embedding medium, frozen, and sections made and mounted on slides. After storage at –70° for 2 days to allow the antigen sections to become firmly affixed to the slides, they may be used to perform the IFA test for trichinosis. This method is less time-consuming, due to the elimination of centrifugation steps, than the antigen presently in use for the trichinosis IFA test, and should be applicable to other IFA procedures.
制备了一种稳定的表皮玻片抗原，用于旋毛虫病的间接荧光抗体（IFA）检测。通过胃蛋白酶消化制备的幼虫表皮，嵌入Tissue-Tek OCT包埋介质中，冷冻，切片并固定在玻片上。在-70°储存2天，使抗原切片牢固地附着在玻片上后，即可用于进行旋毛虫病的IFA检测。与目前用于旋毛虫病IFA检测的抗原相比，该方法由于省去了离心步骤，耗时更少，并且应适用于其他IFA程序。
