Thomas R, Aikawa N, Burke J F
Surgery. 1979 Nov;86(5):742-7.
The glucose uptake of a perfused, skin-covered preparation of muscle taken from the rat was altered by prior burn injury to the animal. Animals receiving an 8% deep burn to the back 24 hours prior to testing had a glucose uptake (5.9 +/- 0.85 microM/100 gm/min) which was depressed compared with control nonburned preparations (8.4 +/- 0.34 microM/100 gm/min) (P less than 0.025) at low insulin concentration (35 microU/ml). Higher insulin concentrations (160 microU/ml and 16 microU/ml) produced significant increases in the glucose uptake of both the burned and control animals. At the highest insulin level (16 microU/ml), there was no significant difference between burned and control animals. Preparations from animals burned five days prior to study showed a glucose uptake which was not decreased as compared with control animals at the low level of insulin (35 microU/ml) (7.4 +/- 0.62 microM/100 gm/min), but higher levels of insulin (160 microU/ml and 16 microU/ml) failed to produce a significant increase in glucose uptake (8.4 +/- 0.64 microM/100 gm/min and 8.6 +/- 0.92 microM/100 gm/min, respectively). The values differed significantly (P less than 0.05) from control preparation (11.5 +/- 0.54 microM/100 gm/min) at the insulin level of 16 microU/ml. Two different patterns of altered sensitivity to increased insulin concentrations were noted, depending on the stage of injury. The altered metabolic status of peripheral tissues does contribute to the insulin resistance pheonomenon observed after a burn injury.
对大鼠进行预先烧伤会改变从其身上获取的带皮肤灌注肌肉制剂的葡萄糖摄取。在测试前24小时接受背部8%深度烧伤的动物，在低胰岛素浓度（35微单位/毫升）下，其葡萄糖摄取量（5.9±0.85微摩尔/100克/分钟）与未烧伤的对照制剂（8.4±0.34微摩尔/100克/分钟）相比有所降低（P＜0.025）。较高的胰岛素浓度（160微单位/毫升和16微单位/毫升）会使烧伤和对照动物的葡萄糖摄取量显著增加。在最高胰岛素水平（16微单位/毫升）时，烧伤动物和对照动物之间没有显著差异。在研究前五天烧伤的动物的制剂，在低胰岛素水平（35微单位/毫升）下，其葡萄糖摄取量与对照动物相比没有降低（7.4±0.62微摩尔/100克/分钟），但较高水平的胰岛素（160微单位/毫升和16微单位/毫升）未能使葡萄糖摄取量显著增加（分别为8.4±0.64微摩尔/100克/分钟和8.6±0.92微摩尔/100克/分钟）。在胰岛素水平为16微单位/毫升时，这些值与对照制剂（11.5±0.54微摩尔/100克/分钟）有显著差异（P＜0.05）。根据损伤阶段的不同，观察到两种对胰岛素浓度升高的敏感性改变模式。外周组织代谢状态的改变确实导致了烧伤后观察到的胰岛素抵抗现象。
