尿苷二磷酸-4-酮基葡萄糖，尿苷二磷酸-半乳糖-4-差向异构酶反应中的一种中间产物。
Uridine diphosphate-4-keto-glucose, an intermediate in the uridine diphosphate-galactose-4-epimerase reaction.
作者信息
Maitra U S, Ankel H
出版信息
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2660-3. doi: 10.1073/pnas.68.11.2660.
Abstract
When UDP-galactose 4-epimerase (EC 5.1.3.2) from Escherichia coli is incubated with UDP-galactose, then reduced with NaB(3)H(4), label is found in UDP-glucose and UDP-galactose. Enzymatic and chemical degradation demonstrates that the label is bound to carbon 4 of the glycosyl moieties. These results provide direct evidence for the existence of UDP-4-keto-glucose as an enzyme-bound intermediate in the epimerization reaction, and they exclude the formation of a 3-keto intermediate.
摘要
当将来自大肠杆菌的UDP - 半乳糖4 - 表异构酶（EC 5.1.3.2）与UDP - 半乳糖一起温育，然后用NaB(3)H(4)还原时，在UDP - 葡萄糖和UDP - 半乳糖中发现有放射性标记。酶促降解和化学降解表明该标记与糖基部分的碳4结合。这些结果为UDP - 4 - 酮基 - 葡萄糖作为表异构化反应中酶结合中间体的存在提供了直接证据，并且排除了3 - 酮基中间体的形成。
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