大鼠肝脏细胞内血清白蛋白的分离
The separation of intracellular serum albumin from rat liver.
作者信息
Judah J D, Nicholls M R
出版信息
Biochem J. 1971 Jul;123(4):643-8. doi: 10.1042/bj1230643.
Abstract
- Antibody precipitation of serum albumin from rat liver extracts yields impure preparations of the protein. 2. When rat liver is labelled with l-[1-(14)C]leucine, antibody precipitation of albumin leads to material that is contaminated with a protein or proteins of very high specific radioactivity. Only 10-25% of the radioactivity of the antibody precipitate is associated with serum albumin. 3. A chromatographic procedure is described that can be used to separate radiochemically pure serum albumin from antibody precipitates obtained from extracts of rat liver. 4. Extracellular albumin secreted by liver slices yields a precipitate with antibody which contains much less radioactive impurity. About 70-90% of the radioactivity is associated with serum albumin. Serum albumin separated by antibody precipitation from rat serum labelled in vivo was not contaminated with the radiochemical impurities associated with intracellular albumin. 5. A simple method is described of obtaining the content of serum albumin in rat liver extracts by the technique of isotope dilution and ion-exchange chromatography.
摘要
- 用抗体从大鼠肝脏提取物中沉淀血清白蛋白会得到不纯的蛋白质制剂。2. 当用L-[1-(14)C]亮氨酸标记大鼠肝脏时，白蛋白的抗体沉淀会导致物质被具有非常高比放射性的一种或多种蛋白质污染。抗体沉淀物中只有10%-25%的放射性与血清白蛋白相关。3. 描述了一种色谱方法，可用于从大鼠肝脏提取物的抗体沉淀物中分离出放射化学纯的血清白蛋白。4. 肝切片分泌的细胞外白蛋白与抗体产生的沉淀物含有少得多的放射性杂质。约70%-90%的放射性与血清白蛋白相关。通过抗体沉淀从体内标记的大鼠血清中分离出的血清白蛋白没有被与细胞内白蛋白相关的放射化学杂质污染。5. 描述了一种通过同位素稀释和离子交换色谱技术获得大鼠肝脏提取物中血清白蛋白含量的简单方法。
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引用本文的文献
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Biosynthesis of rat serum albumin.大鼠血清白蛋白的生物合成
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3
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本文引用的文献
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Measurement of intracellular albumin in rat liver.大鼠肝脏细胞内白蛋白的测量。
Biochem J. 1961 Mar;78(3):551-6. doi: 10.1042/bj0780551.
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The determination of serum albumin and globulin by a new method.用一种新方法测定血清白蛋白和球蛋白。
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Biochem J. 1957 May;66(1):19-31. doi: 10.1042/bj0660019.
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8
The proption of the incorporation of leucine into albumin to that into totaprotein in rat liver and hepatoma Morris 5123 TC.大鼠肝脏和肝癌莫里斯5123 TC中亮氨酸掺入白蛋白与掺入总蛋白的比例。
Eur J Biochem. 1969 Sep;10(2):355-61. doi: 10.1111/j.1432-1033.1969.tb00698.x.
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