Taura S, Taura M, Imai H, Kummerow F A
Tohoku J Exp Med. 1979 Sep;129(1):25-39. doi: 10.1620/tjem.129.25.
The aortic endothelium in weanling swine was rubbed ten times with an inflated balloon catheter in a repeated balloon denudation. This procedure produced more drastic, extensive and uniform changes in the aortic wall than the commonly used balloon denudation. Sequential alternations of regenerating endothelium, intimal thickening and medial reaction, and the incidence of mitotic cells were studied by scanning and transmission electron microscopy. Complete endothelial loss was confirmed from the descending thoracic aorta to the right femoral artery within 24 hr. By the third day, regenerated endothelium began to cover over the endothelial defect area from the uninjured areas such as the aortic arch and the orifices of branching arteries. Thrombus formation and fibromuscular intimal thickening were observed in the endothelial defect areas by the fifth and seventh days. Three types of mitotic cells, such as endothelial, intimal and medial cells were noted in the aortic wall. Mitotic endothelial and mitotic medial cells were most frequent at Day 3; the latter were closely associated with dead medial cells. Mitotic intimal cells initially appeared at Day 3 and were most frequent at Day 7. Mitotic intimal and mitotic medial cells were frequently present in the aortic wall subjacent to the endothelial defect areas containing interstitial blood components. The large numbers of mitotic aortic cells indicated that endothelial cells give rise to new endothelial cells, intimal cells to new intimal cells, and medial cells to new medial cells.
在断乳仔猪中，使用充气的球囊导管对主动脉内皮进行反复球囊剥脱，共摩擦十次。与常用的球囊剥脱相比，该操作在主动脉壁上产生了更剧烈、广泛且均匀的变化。通过扫描电子显微镜和透射电子显微镜研究了再生内皮、内膜增厚和中膜反应的连续变化以及有丝分裂细胞的发生率。在24小时内，从胸降主动脉到右股动脉均证实内皮完全缺失。到第三天，再生内皮开始从主动脉弓和分支动脉开口等未受损区域覆盖内皮缺损区域。在第五天和第七天，在内皮缺损区域观察到血栓形成和纤维肌性内膜增厚。在主动脉壁中发现了三种有丝分裂细胞，即内皮细胞、内膜细胞和中膜细胞。有丝分裂的内皮细胞和有丝分裂的中膜细胞在第3天最为常见；后者与死亡的中膜细胞密切相关。有丝分裂的内膜细胞最初出现在第3天，在第7天最为常见。有丝分裂的内膜细胞和有丝分裂的中膜细胞经常出现在含有间质血液成分的内皮缺损区域下方的主动脉壁中。大量有丝分裂的主动脉细胞表明，内皮细胞可产生新的内皮细胞，内膜细胞可产生新的内膜细胞，中膜细胞可产生新的中膜细胞。
