Reidy M A, Standaert D, Schwartz S M
Arteriosclerosis. 1982 May-Jun;2(3):216-20. doi: 10.1161/01.atv.2.3.216.
Aortas of male rabbits (2.0 to 2.5 kg body weight) were denuded of endothelium with a 4F balloon catheter and killed 7, 14, 60, 90, and 180 days later. Aortic endothelial cells regrowth was determined using Evans blue dye and scanning electron microscopy. Early endothelial outgrowth was rapid, but by 14 days the regrowth slowed markedly so that no further increase of endothelial outgrowth was detected at other time periods. Another group of animals received 3H-thymidine 1 hour before death and were killed 4, 14, and 42 days later. The endothelial cells replication in the aortas of these animals was measured from Häutchen preparations. Endothelial cells replication was observed 4 days after balloon injury but was markedly reduced by 14 and 42 days. Intimal smooth muscle cell replication, however, was observed at both these later times. These result demonstrate that in vivo endothelial cells replication stops long before the aorta is repopulated and suggest that some mechanism other than contact between endothelial cells can prevent endothelial cell replication.
选用体重2.0至2.5千克的雄性兔，用4F球囊导管剥脱其主动脉内皮，分别于7天、14天、60天、90天和180天后处死。采用伊文思蓝染料和扫描电子显微镜检测主动脉内皮细胞的再生情况。内皮细胞早期生长迅速，但到14天时，再生明显减缓，以至于在其他时间段未检测到内皮细胞生长的进一步增加。另一组动物在处死前1小时接受3H-胸腺嘧啶核苷，分别于4天、14天和42天后处死。从Häutchen标本中测量这些动物主动脉内皮细胞的复制情况。在球囊损伤后4天观察到内皮细胞复制，但在14天和42天时明显减少。然而，在这两个较晚的时间均观察到内膜平滑肌细胞的复制。这些结果表明，在体内，主动脉重新内皮化之前很久内皮细胞复制就已停止，提示除内皮细胞之间的接触外，某些其他机制可阻止内皮细胞复制。
