Nairn R C, Nind A P, Guli E P, Davies D J, Rolland J M, McGiven A R, Hughes E S
Br Med J. 1971 Dec 18;4(5789):706-9. doi: 10.1136/bmj.4.5789.706.
Sixty cases of colonic carcinoma have been investigated for antitumour immunoreactivity. The tests employed were blood lymphocyte reactivity and complement-dependent serum cytotoxicity against cultured tumour cells, and immunofluorescence for membrane staining of viable tumour cells and cytoplasmic staining of dried tumour cells in films. Nineteen cases were positive by one or more tests and the most frequent positive response, lymphocytotoxicity, was detected in 8 of the 24 cases tested in this way. The lymphocytotoxicity persisted in a case tested three times over a year. Immunoreactivity against tumour cell surface, as by lymphocyte or serum cytotoxicity or membrane immunofluorescence, was restricted to colonic carcinomas but there was an additional element of individual specificity; cross-reactivity with other tissues and tumours was not observed. Lymphocytes from regional lymph nodes were non-reactive even in a case with positive blood lymphocyte cytotoxicity against the carcinoma cells.
对60例结肠癌患者进行了抗肿瘤免疫反应性研究。所采用的检测方法包括血液淋巴细胞反应性、针对培养肿瘤细胞的补体依赖性血清细胞毒性，以及对活肿瘤细胞膜染色和对薄膜中干燥肿瘤细胞进行细胞质染色的免疫荧光法。19例患者通过一项或多项检测呈阳性，最常见的阳性反应——淋巴细胞毒性，在以这种方式检测的24例患者中有8例出现。淋巴细胞毒性在一年内接受三次检测的一名患者中持续存在。通过淋巴细胞或血清细胞毒性或膜免疫荧光检测到的针对肿瘤细胞表面的免疫反应性仅限于结肠癌，但还存在个体特异性的额外因素；未观察到与其他组织和肿瘤的交叉反应。即使在血液淋巴细胞对癌细胞具有阳性细胞毒性的病例中，区域淋巴结中的淋巴细胞也无反应。
