Mackay G R
Stain Technol. 1979 Mar;54(2):85-7. doi: 10.3109/10520297909112639.
A modification of a scanning transmission electron microscope specimen holder which permits full viewing of large plastic embedded tissue sections is discussed. The method for producing one-centimeter diameter "giant" grids is explained and the procedure for sample preparation is outlined. The modification aids the microscopist in his evaluation of tissue structural relationships by providing large areas of tissue for examination and reduces significantly the time required to prepare and examine standard 1-2 mm2 electron microscopy tissue sections. Light and electron microscopic evaluations can be made on the same tissue sections.
本文讨论了一种扫描透射电子显微镜样品架的改进方法，该方法可实现对大型塑料包埋组织切片的全面观察。文中解释了制备直径为一厘米的“巨型”网格的方法，并概述了样品制备过程。这种改进为显微镜学家评估组织结构关系提供了大面积的组织供检查，有助于其工作，同时显著减少了制备和检查标准1-2平方毫米电子显微镜组织切片所需的时间。光镜和电镜评估可在同一切片上进行。
