牛胃蛋白酶原和胃蛋白酶。活性天冬氨酰残基周围的序列。
Bovine pepsinogens and pepsins. The sequence around a reactive aspartyl residue.
作者信息
Meitner P A
出版信息
Biochem J. 1971 Oct;124(4):673-6. doi: 10.1042/bj1240673.
Abstract
The specific inhibitor, N-diazoacetylnorleucine methyl ester reacts stoicheiometrically with bovine pepsin resulting in a simultaneous loss of all enzymic activity. A peptide containing a modified aspartyl group was isolated from bovine pepsin labelled with (14)C-labelled inhibitor. The aspartic acid residue is presumed to be part of the active centre and is in the same heptapeptide sequence as in porcine pepsin: Ile-Val-Asp-Thr-Gly-Thr-Ser.
摘要
特异性抑制剂N-重氮乙酰基正亮氨酸甲酯与牛胃蛋白酶按化学计量反应，导致所有酶活性同时丧失。从用（14）C标记的抑制剂标记的牛胃蛋白酶中分离出一种含有修饰天冬氨酰基的肽。天冬氨酸残基被认为是活性中心的一部分，并且与猪胃蛋白酶中的七肽序列相同：异亮氨酸-缬氨酸-天冬氨酸-苏氨酸-甘氨酸-苏氨酸-丝氨酸。
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本文引用的文献
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Specific inactivation of pepsin by a diazo ketone.重氮酮对胃蛋白酶的特异性失活作用。
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Stereochemical investigation of the active center of pepsin using a new inactivator.使用新型失活剂对胃蛋白酶活性中心进行立体化学研究。
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The inactivation of pepsin by diazoacetylnorleucine methyl ester.重氮乙酰正亮氨酸甲酯对胃蛋白酶的失活作用。
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Inactivation of pepsin by diphenyldiazomethane.二苯基重氮甲烷对胃蛋白酶的灭活作用。
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