酸性蛋白酶抑制剂对鸡胃蛋白酶的作用。
The effect of acid proteinase inhibitors on chicken pepsin.
作者信息
Llewellin J M, Green M L
出版信息
Biochem J. 1975 Nov;151(2):319-26. doi: 10.1042/bj1510319.
Abstract
- The activity of chicken pepsin was partially inhibited by dimethyl-(2-hydroxy-5-nitrobenzyl)sulphonium bromide, but was unaffected by p-bromophenacyl bromide. 2. In the presence of Cu2+, diazoacetylnorleucine methyl ester completely inactivated chicken pepsin with the incorporation of 1 mol/mol. The mechanism of the reaction was similar to that with pig pepsin. 3. Chicken pepsin was completely inactivated by 2-diazo-4-bromoacetophenone in the presence of Cu2+. 4. Chicken pepsin was almost completely inactivated by 1,2-epoxy-3-(p-nitrophenoxy)propane at 25 degrees C, 3-4mol of inhibitor/mol being incorporated. The reaction at 10 degrees C was investigated briefly. 5. Calf chymosin was inactivated by 1,2-epoxy-3-(p-nitrophenoxy)propane at 10 degrees C, the incorporation of 1 mol/mol being required for complete inhibition. 6. The characteristics of the reactions of chicken pepsin with the above compounds were compared with those of other acid proteinases.
摘要
- 二甲基 -（2 - 羟基 - 5 - 硝基苄基）溴化锍对鸡胃蛋白酶的活性有部分抑制作用，但对对溴苯甲酰溴不敏感。2. 在Cu2 +存在下，重氮乙酰 - 正亮氨酸甲酯能使鸡胃蛋白酶完全失活，每摩尔酶结合1摩尔抑制剂。该反应机制与猪胃蛋白酶的相似。3. 在Cu2 +存在下，2 - 重氮 - 4 - 溴苯乙酮能使鸡胃蛋白酶完全失活。4. 在25℃时，1,2 - 环氧 - 3 -（对硝基苯氧基）丙烷能使鸡胃蛋白酶几乎完全失活，每摩尔酶结合3 - 4摩尔抑制剂。对10℃时的反应进行了简要研究。5. 在10℃时，1,2 - 环氧 - 3 -（对硝基苯氧基）丙烷能使小牛凝乳酶失活，完全抑制需要每摩尔酶结合1摩尔抑制剂。6. 将鸡胃蛋白酶与上述化合物反应的特性与其他酸性蛋白酶的特性进行了比较。
相似文献
1
The effect of acid proteinase inhibitors on chicken pepsin.酸性蛋白酶抑制剂对鸡胃蛋白酶的作用。
Biochem J. 1975 Nov;151(2):319-26. doi: 10.1042/bj1510319.
2
The structure and function of acid proteases. V. Comparative studies on the specific inhibition of acid proteases by diazoacetyl-DL-norleucine methyl ester, 1,2-epoxy-3-(p-nitrophenoxy) propane and pepstatin.酸性蛋白酶的结构与功能。V. 重氮乙酰-DL-正亮氨酸甲酯、1,2-环氧-3-(对硝基苯氧基)丙烷和胃蛋白酶抑制剂对酸性蛋白酶特异性抑制作用的比较研究。
J Biochem. 1976 Sep;80(3):497-506. doi: 10.1093/oxfordjournals.jbchem.a131304.
3
The structure and function of acid proteases. VIII. Purification and characterization of cathepsins D from Japanese monkey lung.酸性蛋白酶的结构与功能。VIII. 日本猕猴肺组织中组织蛋白酶D的纯化与特性分析
J Biochem. 1978 Feb;83(2):441-51. doi: 10.1093/oxfordjournals.jbchem.a131931.
4
Pepsinogen C and pepsin C from gastric mucosa of Japanese monkey. Purification and characterization.日本猕猴胃黏膜中的胃蛋白酶原C和胃蛋白酶C。纯化与特性分析。
J Biochem. 1976 Nov;80(5):983-92. doi: 10.1093/oxfordjournals.jbchem.a131386.
5
Inhibition of the acid proteinase from Neurospora crassa by diazoacetyl-DL-norleucine methyl ester, 1,2-epoxy-3-(4-nitrophenoxy)propane and pepstatin.重氮乙酰-DL-正亮氨酸甲酯、1,2-环氧-3-(4-硝基苯氧基)丙烷和胃蛋白酶抑制剂对粗糙脉孢菌酸性蛋白酶的抑制作用
Hoppe Seylers Z Physiol Chem. 1979 Jun;360(6):791-4.
6
7
The structure and function of acid proteases. VI. Effects of acid protease-specific inhibitors on the acid proteases from Aspergillus niger var. macrosporus.酸性蛋白酶的结构与功能。VI. 酸性蛋白酶特异性抑制剂对黑曲霉大孢子变种酸性蛋白酶的影响。
J Biochem. 1976 Nov;80(5):975-81. doi: 10.1093/oxfordjournals.jbchem.a131385.
8
Ostrich pepsins I and II: a kinetic and thermodynamic investigation.鸵鸟胃蛋白酶I和II：动力学与热力学研究
Int J Biochem Cell Biol. 1995 Dec;27(12):1293-302. doi: 10.1016/1357-2725(95)00092-4.
9
Characterisation of a thermostable pepstatin-insensitive acid proteinase from a Bacillus sp.一株芽孢杆菌属菌株中一种对胃蛋白酶抑制剂不敏感的耐热酸性蛋白酶的特性分析
Int J Biochem Cell Biol. 1995 Jul;27(7):729-39. doi: 10.1016/1357-2725(95)00032-k.
10
The structure and function of acid proteases. IV. Inactivation of the acid protease from Mucor pusillus by acid protease-specific inhibitors.酸性蛋白酶的结构与功能。IV. 特异性酸性蛋白酶抑制剂对微小毛霉酸性蛋白酶的失活作用
J Biochem. 1976 Jul;80(1):61-7. doi: 10.1093/oxfordjournals.jbchem.a131258.
引用本文的文献
1
alpha-Bromo-4-amino-3-nitroacetophenone, a new reagent for protein modification. Modification of the methionine-290 residue of porcine pepsin.α-溴-4-氨基-3-硝基苯乙酮，一种用于蛋白质修饰的新试剂。猪胃蛋白酶甲硫氨酸-290残基的修饰
Biochem J. 1980 May 1;187(2):345-52. doi: 10.1042/bj1870345.
2
Crystallization of one of the chicken pepinogens and its derived pepsin.一种鸡胃蛋白酶原及其衍生的胃蛋白酶的结晶
Biochem J. 1975 Dec;151(3):763-4. doi: 10.1042/bj1510763.
本文引用的文献
1
THE ACTION OF RENNIN ON CASEIN: THE EFFECT OF MODIFYING FUNCTIONAL GROUPS ON THE RENNIN.凝乳酶对酪蛋白的作用：修饰凝乳酶上功能基团的影响。
Biochim Biophys Acta. 1965 May 18;99:352-9. doi: 10.1016/s0926-6593(65)80132-1.
2
A MICRO-BIURET METHOD FOR ESTIMATING PROTEINS.一种用于蛋白质定量的微量双缩脲法。
Anal Biochem. 1964 Dec;9:401-10. doi: 10.1016/0003-2697(64)90200-3.
3
[THE PREPARATION AND CERTAIN PROPERTIES OF CHICK PEPSIN].[鸡胃蛋白酶的制备及其某些性质]
Biokhimiia. 1963 Nov-Dec;28:1004-10.
4
The primary structure of prochymosin (prorennin) EC 3.4.4.3. some tryptic fragments of a maleylated preparation.凝乳酶原（前肾素）EC 3.4.4.3的一级结构。一种马来酰化制剂的一些胰蛋白酶片段。
FEBS Lett. 1971 Sep 15;17(1):87-89. doi: 10.1016/0014-5793(71)80570-7.
5
A colorimetric procedure for the quantitative determination of tryptophan residues in proteins.一种用于定量测定蛋白质中色氨酸残基的比色法。
J Biol Chem. 1967 Dec 25;242(23):5771-6.
6
Amino acid sequence around the active site aspartic acid in penicillopepsin.青霉胃蛋白酶活性位点天冬氨酸周围的氨基酸序列。
Can J Biochem. 1970 Sep;48(9):1014-6. doi: 10.1139/o70-158.
7
Stereochemical investigation of the active center of pepsin using a new inactivator.使用新型失活剂对胃蛋白酶活性中心进行立体化学研究。
Biochem Biophys Res Commun. 1967 Jul 21;28(2):203-8. doi: 10.1016/0006-291x(67)90430-5.
8
The inactivation of pepsin by diazoacetylnorleucine methyl ester.重氮乙酰正亮氨酸甲酯对胃蛋白酶的失活作用。
J Biol Chem. 1966 Sep 25;241(18):4295-7.
9
Bovine pepsinogens and pepsins. The sequence around a reactive aspartyl residue.牛胃蛋白酶原和胃蛋白酶。活性天冬氨酰残基周围的序列。
Biochem J. 1971 Oct;124(4):673-6. doi: 10.1042/bj1240673.
10
An active site peptide from pepsin C.胃蛋白酶C的活性位点肽段。
Biochem J. 1971 Jun;123(1):75-82. doi: 10.1042/bj1230075.
Zotero 插件