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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Llewellin J M, Green M L

Biochem J. 1975 Nov;151(2):319-26. doi: 10.1042/bj1510319.

The activity of chicken pepsin was partially inhibited by dimethyl-(2-hydroxy-5-nitrobenzyl)sulphonium bromide, but was unaffected by p-bromophenacyl bromide. 2. In the presence of Cu2+, diazoacetylnorleucine methyl ester completely inactivated chicken pepsin with the incorporation of 1 mol/mol. The mechanism of the reaction was similar to that with pig pepsin. 3. Chicken pepsin was completely inactivated by 2-diazo-4-bromoacetophenone in the presence of Cu2+. 4. Chicken pepsin was almost completely inactivated by 1,2-epoxy-3-(p-nitrophenoxy)propane at 25 degrees C, 3-4mol of inhibitor/mol being incorporated. The reaction at 10 degrees C was investigated briefly. 5. Calf chymosin was inactivated by 1,2-epoxy-3-(p-nitrophenoxy)propane at 10 degrees C, the incorporation of 1 mol/mol being required for complete inhibition. 6. The characteristics of the reactions of chicken pepsin with the above compounds were compared with those of other acid proteinases.

二甲基 -（2 - 羟基 - 5 - 硝基苄基）溴化锍对鸡胃蛋白酶的活性有部分抑制作用，但对对溴苯甲酰溴不敏感。2. 在Cu2 +存在下，重氮乙酰 - 正亮氨酸甲酯能使鸡胃蛋白酶完全失活，每摩尔酶结合1摩尔抑制剂。该反应机制与猪胃蛋白酶的相似。3. 在Cu2 +存在下，2 - 重氮 - 4 - 溴苯乙酮能使鸡胃蛋白酶完全失活。4. 在25℃时，1,2 - 环氧 - 3 -（对硝基苯氧基）丙烷能使鸡胃蛋白酶几乎完全失活，每摩尔酶结合3 - 4摩尔抑制剂。对10℃时的反应进行了简要研究。5. 在10℃时，1,2 - 环氧 - 3 -（对硝基苯氧基）丙烷能使小牛凝乳酶失活，完全抑制需要每摩尔酶结合1摩尔抑制剂。6. 将鸡胃蛋白酶与上述化合物反应的特性与其他酸性蛋白酶的特性进行了比较。

Llewellin J M, Green M L

Biochem J. 1975 Nov;151(2):319-26. doi: 10.1042/bj1510319.

The activity of chicken pepsin was partially inhibited by dimethyl-(2-hydroxy-5-nitrobenzyl)sulphonium bromide, but was unaffected by p-bromophenacyl bromide. 2. In the presence of Cu2+, diazoacetylnorleucine methyl ester completely inactivated chicken pepsin with the incorporation of 1 mol/mol. The mechanism of the reaction was similar to that with pig pepsin. 3. Chicken pepsin was completely inactivated by 2-diazo-4-bromoacetophenone in the presence of Cu2+. 4. Chicken pepsin was almost completely inactivated by 1,2-epoxy-3-(p-nitrophenoxy)propane at 25 degrees C, 3-4mol of inhibitor/mol being incorporated. The reaction at 10 degrees C was investigated briefly. 5. Calf chymosin was inactivated by 1,2-epoxy-3-(p-nitrophenoxy)propane at 10 degrees C, the incorporation of 1 mol/mol being required for complete inhibition. 6. The characteristics of the reactions of chicken pepsin with the above compounds were compared with those of other acid proteinases.

二甲基 -（2 - 羟基 - 5 - 硝基苄基）溴化锍对鸡胃蛋白酶的活性有部分抑制作用，但对对溴苯甲酰溴不敏感。2. 在Cu2 +存在下，重氮乙酰 - 正亮氨酸甲酯能使鸡胃蛋白酶完全失活，每摩尔酶结合1摩尔抑制剂。该反应机制与猪胃蛋白酶的相似。3. 在Cu2 +存在下，2 - 重氮 - 4 - 溴苯乙酮能使鸡胃蛋白酶完全失活。4. 在25℃时，1,2 - 环氧 - 3 -（对硝基苯氧基）丙烷能使鸡胃蛋白酶几乎完全失活，每摩尔酶结合3 - 4摩尔抑制剂。对10℃时的反应进行了简要研究。5. 在10℃时，1,2 - 环氧 - 3 -（对硝基苯氧基）丙烷能使小牛凝乳酶失活，完全抑制需要每摩尔酶结合1摩尔抑制剂。6. 将鸡胃蛋白酶与上述化合物反应的特性与其他酸性蛋白酶的特性进行了比较。