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一种需要细胞壁半乳糖的大肠杆菌噬菌体的分离与鉴定

Isolation and characterization of an Escherichia coli bacteriophage requiring cell wall galactose.

作者信息

Watson G, Paigen K

出版信息

J Virol. 1971 Nov;8(5):669-74. doi: 10.1128/JVI.8.5.669-674.1971.

Abstract

A new coliphage, designated U3, has been selected for the ability to discriminate the presence of galactose in the cell wall of Escherichia coli. U3 attacks E. coli K-12 cells that are able to incorporate galactose into their cell walls, but mutants blocked in the synthesis of uridine diphosphogalactose, the precursor of cell wall galactose, are completely resistant to the phage. U3 is a small, tail-less, approximately spherical phage resembling phiX174 in its physical properties. Its diameter by electron microscopy is 21 to 22 nm, and its particle weight is approximately 4 x 10(6) daltons. Like phiX174, U3 appears to have a single-stranded deoxyribonucleic acid genome and has at least four cistrons.

摘要

一种新的大肠杆菌噬菌体,命名为U3,因其能够识别大肠杆菌细胞壁中半乳糖的存在而被挑选出来。U3攻击能够将半乳糖掺入其细胞壁的大肠杆菌K-12细胞,但在细胞壁半乳糖前体尿苷二磷酸半乳糖合成过程中受阻的突变体对该噬菌体完全具有抗性。U3是一种小型、无尾、近似球形的噬菌体,其物理性质类似于φX174。通过电子显微镜观察,其直径为21至22纳米,颗粒重量约为4×10⁶道尔顿。与φX174一样,U3似乎具有单链脱氧核糖核酸基因组,并且至少有四个顺反子。

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