Khokhlov A N, Vilenchik M M
Tsitologiia. 1979 Aug;21(8):986-8.
A device is described providing for lysis of cells grown on glass slides, directly on the top of sucrose gradient. The device allows to avoid cell removing with trypsin, EDTA or due to scraping. The possibility to use this method in studying DNA damage and repair processes in cultured animal cells has been shown using diploid human fibroblasts.
描述了一种装置，用于直接在蔗糖梯度顶部裂解生长在载玻片上的细胞。该装置可避免使用胰蛋白酶、乙二胺四乙酸（EDTA）或刮擦来去除细胞。使用二倍体人成纤维细胞已证明了在研究培养的动物细胞中的DNA损伤和修复过程中使用这种方法的可能性。
