离心式血小板单采术中新型浓度枸橼酸盐抗凝剂的应用。III. 体外血小板代谢评估。

The utilisation of a new strength citrate anticoagulant during centrifugal plateletpheresis. III. Assessment of in vitro platelet metabolism.

作者信息

Zitko M, Reuter H, Borberg H, Mishler J M

出版信息

Vox Sang. 1979;36(6):347-52. doi: 10.1111/j.1423-0410.1979.tb04509.x.

DOI:10.1111/j.1423-0410.1979.tb04509.x

Abstract

Employing the Haemonetics Blood Processor (IFC), a relatively pure platelet concentrate can be prepared by collecting only the first portion of the PRP leaving the centrifuge bowl (Fraction I). A subsequent fraction containing RBC and WBC contaminants (Fraction II) can be purified by means of a second centrifugation, using a conventional blood bank centrifuge (Fraction II), if transfusion of these contaminants would be detrimental to the recipient. Utilising the new 1.4% Na3-citrate anticoagulant, platelet metabolic parameters (ATP, ADP, AMP, lactate and pyruvate) as well as O2-uptake, were determined in Fraction I and II prepared from 10 normal healthy subjects. In contrast to previous studies reporting marked dysfunction in platelets contained in Fraction II when standard ACD-A was used during IFC, we observed no significant difference (Student's t test) in the present study between Fractions I and II, in regard to platelet metabolism, when using the new anticoagulant. It is further concluded that the second centrifugal manipulation does not exert a detrimental effect on platelet metabolism.

摘要

使用Haemonetics血液处理器（IFC），通过仅收集离开离心碗的富血小板血浆（PRP）的第一部分（组分I），可以制备相对纯净的血小板浓缩物。如果这些污染物的输注对受者有害，则可以使用传统血库离心机对含有红细胞和白细胞污染物的后续部分（组分II）进行第二次离心纯化（组分II）。利用新的1.4%柠檬酸钠抗凝剂，对10名正常健康受试者制备的组分I和组分II中的血小板代谢参数（ATP、ADP、AMP、乳酸和丙酮酸）以及氧气摄取进行了测定。与之前的研究不同，之前的研究报告称，在IFC过程中使用标准ACD-A时，组分II中的血小板存在明显功能障碍，而在本研究中，当使用新的抗凝剂时，我们观察到组分I和组分II在血小板代谢方面没有显著差异（学生t检验）。进一步得出结论，第二次离心操作不会对血小板代谢产生有害影响。