Suzuki J B, Grecz N, Windhorst D
Infect Immun. 1971 Sep;4(3):232-6. doi: 10.1128/iai.4.3.232-236.1971.
Leukocytes from two patients with chronic granulomatous disease of childhood (CGD) and from one female carrier were examined for their capacity to induce germination of Clostridium botulinum type A spores in vitro. Normal human leukocytes induced germination of C. botulinum spores to the same extent as guinea pig neutrophils. Germination was depressed by more than half when cells from CGD patients were used. A noticeable, but less severe, abnormality was present in leukocytes from a female carrier for this X-linked trait. CGD leukocytes are defective in cellular production of H(2)O(2), and the latter is known to be effective in inducing germination of clostridial spores. Lysozyme is also known to be effective in spore germination and is present in CGD leukocytes in normal amounts. The posibility that C. botulinum spores require a mechanism which sensitizes them to the action of H(2)O(2) and lysozyme is raised, and it is suggested that the methodology used in this work measures a defect of such a mechanism in CGD leukocytes.
对两名患有儿童慢性肉芽肿病（CGD）的患者以及一名女性携带者的白细胞进行了检测，以评估它们在体外诱导A型肉毒梭菌孢子萌发的能力。正常人的白细胞诱导肉毒梭菌孢子萌发的程度与豚鼠嗜中性粒细胞相同。当使用CGD患者的细胞时，萌发率降低了一半以上。对于这种X连锁性状的女性携带者，其白细胞存在明显但不太严重的异常。CGD白细胞在细胞产生过氧化氢（H₂O₂）方面存在缺陷，而后者已知可有效诱导梭菌孢子萌发。溶菌酶也已知对孢子萌发有效，并且在CGD白细胞中的含量正常。由此提出肉毒梭菌孢子可能需要一种使其对H₂O₂和溶菌酶的作用敏感的机制，并且表明这项工作中使用的方法检测到了CGD白细胞中这种机制的缺陷。
