Smeds S, Anderberg B
Acta Endocrinol (Copenh). 1979 Aug;91(4):644-9. doi: 10.1530/acta.0.0910644.
By means of a micropuncture technique and microgel electrophoresis the luminal content of single rat thyroid follicles were analyzed during PTU treatment for 4 weeks. The relative amount of aggregates of thyroglobulin (i.e. the 27S iodoprotein and larger complex) was reduced from 15 per cent to approximately 3 per cent after 48 h. The aggregates were practically absent after 2 weeks. The thyroglobulin fraction was the predominant colloid constituent. The protein concentration of the colloid was not changed at the end of the period of PTU treatment (8.9 g/100 ml). It is concluded that PTU inhibits the aggregation of thyroglobulin to a larger complex. The results further indicate that the aggregates of thyroglobulin are preferentially localized to the periphery of the lumen in the larger follicles.
采用微穿刺技术和微凝胶电泳法，对接受丙硫氧嘧啶（PTU）治疗4周的大鼠单个甲状腺滤泡的腔内内容物进行了分析。在48小时后，甲状腺球蛋白聚集体（即27S碘蛋白和更大的复合物）的相对量从15%降至约3%。2周后聚集体几乎消失。甲状腺球蛋白部分是胶体的主要成分。在PTU治疗期结束时，胶体的蛋白质浓度没有变化（8.9 g/100 ml）。得出的结论是，PTU抑制甲状腺球蛋白聚合成更大的复合物。结果还表明，甲状腺球蛋白聚集体优先定位于较大滤泡腔内的周边。
