Smeds S, Anderberg B
Acta Endocrinol (Copenh). 1977 Feb;84(2):303-13. doi: 10.1530/acta.0.0840303.
A sequential study of the effect of thyroxine (T4) on the protein composition of the colloid in the rat thyroid gland has been carried out in 24 rats. Daily ip injections of 20 mug thyroxine were given and the colloid was analyzed after 1, 2, 3 and 4 days and 1, 2 and 3 weeks. The protein constituents were separated by microgel electrophoresis and measured by microdensitometry. The incorporation of [3H]leucine into the thyroglobulin fraction and into the albumin-like protein in the homogenate of the gland was analyzed after T4 suppression for 2 days. The 2 h thyroidal iodine uptake was analyzed during the first week of T4 administration. During the second and the third day of T4 administration, the amount of 27S and larger complexes of thyroglobulin was reduced by 30-40 per cent and the amount of the albumin-like protein was significantly increased and present in almost all samples. Preliminary data indicated that during the same period the relative amount of [3H]leucine activity was increased in the albumin-like protein fraction compared to the thyroglobulin fraction in the thyroid. It is concluded that the exocytotic vesicles contain the albumin-like protein. The results further indicate that the administration of T4 temporarily changes the relative rates of formation of thyroglobulin and of the albumin-like protein in the follicle.
对24只大鼠进行了一项关于甲状腺素（T4）对大鼠甲状腺胶体蛋白质组成影响的序贯研究。每天腹腔注射20微克甲状腺素，并在1、2、3、4天以及1、2、3周后对胶体进行分析。蛋白质成分通过微凝胶电泳分离，并通过微密度测定法进行测量。在T4抑制2天后，分析了[3H]亮氨酸掺入甲状腺球蛋白部分以及腺体匀浆中类白蛋白的情况。在给予T4的第一周分析了2小时甲状腺碘摄取量。在给予T4的第二天和第三天，甲状腺球蛋白的27S及更大复合物的量减少了30%-40%，类白蛋白的量显著增加，并且几乎在所有样本中都有出现。初步数据表明，在同一时期，与甲状腺中的甲状腺球蛋白部分相比，类白蛋白部分中[3H]亮氨酸活性的相对量增加。结论是胞吐小泡含有类白蛋白。结果还表明，给予T4会暂时改变滤泡中甲状腺球蛋白和类白蛋白的相对形成速率。
