Wilke R, Harmeyer J, von Grabe C, Hehrmann R, Hesch R D
Acta Endocrinol (Copenh). 1979 Oct;92(2):295-308. doi: 10.1530/acta.0.0920295.
A radioimmunoassay for porcine parathyroid hormone has been developed and applied to measure immunoreactive parathyroid hormone (PTH) in plasma of pigs with hereditary vitamin D dependency rickets (VDDR) (pseudovitamin D deficiency rickets). Levels of 25-hydroxycholecalciferol (25-(OH)-D3) in plasma were measured by a protein binding assay. Both plasma concentrations of PTH and 25-(OH)-D3 showed an approximately 4-fold increase compared to normal pigs. PTH levels increased with duration of the disease. Daily dosing of the animals with 1--4 micrograms of 1,25-dihydroxycholecalciferol (1,25-(OH)2-D3) reduced PTH concentrations and resulted in clinical healing. Iv administration of 10 micrograms of 25-(OH)-D3/day did not alter PTH concentrations nor the clinical symptoms. The results suggest that these animals suffer from regulatory hyperparathyroidism. The metabolic defect could be due to a failure of the kidney to convert 25-(OH)-D3 to 1,25-(OH)2-D3.
已开发出一种用于检测猪甲状旁腺激素的放射免疫分析法，并将其应用于测量患有遗传性维生素D依赖性佝偻病（VDDR）（假性维生素D缺乏性佝偻病）的猪血浆中的免疫反应性甲状旁腺激素（PTH）。通过蛋白质结合分析法测量血浆中25-羟基胆钙化醇（25-(OH)-D3）的水平。与正常猪相比，血浆中PTH和25-(OH)-D3的浓度均显示出约4倍的升高。PTH水平随疾病持续时间而增加。给动物每日剂量为1-4微克的1,25-二羟基胆钙化醇（1,25-(OH)2-D3）可降低PTH浓度并导致临床治愈。每天静脉注射10微克的25-(OH)-D3不会改变PTH浓度，也不会改变临床症状。结果表明这些动物患有调节性甲状旁腺功能亢进症。代谢缺陷可能是由于肾脏无法将25-(OH)-D3转化为1,25-(OH)2-D3。
