使用牛血清低分子量激肽原估计尿激肽原酶活性。

Estimation of urinary kininogenase activity using bovine serum low molecular weight kininogen.

作者信息

Abe K, Kato H, Sakurai Y, Itoh T, Saito K, Haruyama T, Otsuka Y, Yoshinaga K

出版信息

Adv Exp Med Biol. 1979;120A:105-14. doi: 10.1007/978-1-4757-0926-1_11.

Abstract

Estimation of urinary kininogenase activity by radioimmunoassay of generated kinin was studied. Bovine serum low molecular weight kininogen was proved not to cross-react with kallidin antibody and also bradykinin antibody. This kininogen was used as substrate measuring urinary kininogenase activity. Separation of released kinin from the kininogen was not required in the present method. Urinary kallikrein activity was found to be significantly decreased in essential hypertension, in chronic glomerulonephritis and in patients who had received renal transplantation. On the contrary, an increase in urinary kallikrein was found in primary aldosteronism and in Bartter's syndrome. The present method was very useful for measuring kininogenase activity.

摘要

通过对生成的激肽进行放射免疫测定来研究尿激肽原酶活性。已证实牛血清低分子量激肽原与胰激肽抗体以及缓激肽抗体均无交叉反应。这种激肽原被用作测量尿激肽原酶活性的底物。在本方法中无需将释放的激肽与激肽原分离。发现在原发性高血压、慢性肾小球肾炎以及接受肾移植的患者中尿激肽释放酶活性显著降低。相反，在原发性醛固酮增多症和巴特综合征中发现尿激肽释放酶增加。本方法对于测量激肽原酶活性非常有用。