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鉴定出两种针对禽脑不同抗原成分的不同兔抗体。

Identification of two distinct rabbit antibodies directed against different antigenic components of avian brain.

作者信息

Friedman H P, Wenger B S

出版信息

Immunology. 1965 Nov;9(5):467-75.

PMID:4954487
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1423718/
Abstract

Two types of antibodies directed against biochemically different antigens have been found in antisera produced by hyperimmunizing rabbits with whole homogenates of chicken or quail brain. DEAE-cellulose column chromatography was used to separate the two antibodies which, based on differences in sedimentation rate and susceptibility to inactivation by 2-mercaptoethanol, were identified as belonging to the 7S and 19S classes respectively. Although both antibodies fix complement in the presence of whole brain homogenates, the 19S fraction reacts only with the chloroform—methanol extractable portion while the 7S fraction reacts only with the residue remaining after such extraction. It is concluded that the 19S and 7S antibodies are directed against lipid or lipoprotein and protein antigens respectively.

摘要

用鸡或鹌鹑脑的全匀浆对家兔进行超免疫所产生的抗血清中，已发现两种针对生化性质不同抗原的抗体。采用二乙氨基乙基纤维素柱色谱法分离这两种抗体，根据沉降速率和对2-巯基乙醇灭活的敏感性差异，分别鉴定它们属于7S和19S类别。虽然两种抗体在全脑匀浆存在时都能固定补体，但19S组分仅与氯仿 - 甲醇可提取部分发生反应，而7S组分仅与这种提取后剩余的残渣发生反应。得出的结论是，19S和7S抗体分别针对脂质或脂蛋白以及蛋白质抗原。

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本文引用的文献

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ON THE DIFFERENT MOLECULAR FORMS OF ANTIBODY SYNTHESIZED BY RABBITS DURING THE EARLY RESPONSE TO A SINGLE INJECTION OF PROTEIN AND CELLULAR ANTIGENS.关于兔子在单次注射蛋白质和细胞抗原后的早期反应中合成的抗体的不同分子形式
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Isolation of brain strandin, a new type of large molecule tissue component.脑链蛋白的分离，一种新型的大分子组织成分。
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Proteolipides, a new type of tissue lipoproteins; their isolation from brain.蛋白脂质，一种新型组织脂蛋白；从脑中分离得到它们。
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THE MOLECULAR PROPERTIES OF ANTIBODIES SYNTHESIZED IN RESPONSE TO SIMPLE HAPTENES.针对简单半抗原产生的抗体的分子特性。
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Autoantibody production in rabbits. II. Organ-specific autontibody in rabbits injected with rat tissues.兔体内自身抗体的产生。II. 注射大鼠组织的兔体内的器官特异性自身抗体。
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Antibrain antibodies of the 19S gamma-globulin type in rats with allergic encephalomyelitis.患有变应性脑脊髓炎的大鼠体内19Sγ-球蛋白类型的抗脑抗体
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Physical properties of antibody to bovine serum albumin as demonstrated by hemagglutination.通过血凝反应所证实的抗牛血清白蛋白抗体的物理性质。
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