Anderson D R, Hopps H E, Barile M F, Bernheim B C
J Bacteriol. 1965 Nov;90(5):1387-404. doi: 10.1128/jb.90.5.1387-1404.1965.
Anderson, Douglas R. (National Cancer Institute, Bethesda, Md.), Hope E. Hopps, Michael F. Barile, and Barbara C. Bernheim. Comparison of the ultrastructure of several rickettsiae, ornithosis virus, and Mycoplasma in tissue culture. J. Bacteriol. 90:1387-1404. 1965.-In an effort to make a valid comparison of the ultrastructure of several intracellular parasites, selected agents were propagated under identical conditions in a single type of tissue culture cell; such infected preparations were processed for examination by electron microscopy by use of a standardized procedure for fixation and embedding. The organisms studied were: the Breinl and E strains of epidemic typhus, Rickettsia prowazeki; the Bitterroot strain of R. rickettsii; the Karp strain of R. tsutsugamushi (R. orientalis); R. sennetsu; the P-4 strain of ornithosis virus; and the HEp-2 strain of Mycoplasma hominis type I. Each of the rickettsial species examined had a cell wall and a plasma membrane, and contained ribosomes and deoxyribonucleic acid (DNA) in a ground substance. However, certain differences were noted. Both strains of R. prowazeki contained numerous intracytoplasmic electron-lucent spherical structures (4 to 10 mmu), not previously described. R. sennetsu, unlike the other rickettsiae, was not free in the host cytoplasm but was always enclosed in a vacuole. R. rickettsii was observed intranuclearly and in digestive organelles of the host cell as well as in the cytoplasm. Cells infected with ornithosis virus contained several forms representing the stages in its life cycle. The "initial bodies," made up of ribosomes and DNA strands, were morphologically similar to the rickettsiae. In cultures infected with M. hominis, most of the cells became large and multinucleate. Although the Mycoplasma organisms were readily cultivated from these cultures, only a few could be found in the electron microscope preparations. These organisms were extracellular and lacked a cell wall, being bound only by a unit membrane. Again, the internal components were ribosomes and DNA strands. Under the uniform preparative conditions employed here, the three groups of organisms were morphologically distinguishable from one another.
安德森，道格拉斯·R.（美国国立癌症研究所，马里兰州贝塞斯达），霍普·E.霍普斯，迈克尔·F.巴里尔，以及芭芭拉·C.伯恩海姆。几种立克次氏体、鸟疫病毒和支原体在组织培养中的超微结构比较。《细菌学杂志》90:1387 - 1404。1965年。——为了对几种细胞内寄生虫的超微结构进行有效比较，将选定的病原体在单一类型的组织培养细胞中于相同条件下进行培养；对这些感染的制剂采用标准化的固定和包埋程序进行处理，以便通过电子显微镜检查。所研究的病原体包括：普氏立克次氏体的布雷恩尔株和E株；立氏立克次氏体的比特鲁特株；恙虫病立克次氏体（东方立克次氏体）的卡尔普株；塞内茨立克次氏体；鸟疫病毒的P - 4株；以及人型支原体I型的HEP - 2株。所检查的每种立克次氏体物种都有细胞壁和质膜，并且在基质中含有核糖体和脱氧核糖核酸（DNA）。然而，也注意到了某些差异。普氏立克次氏体的两个菌株都含有许多胞质内电子透明的球形结构（4至10微米），此前未曾描述过。与其他立克次氏体不同，塞内茨立克次氏体在宿主细胞质中并非游离状态，而是始终被包裹在一个液泡中。立氏立克次氏体在宿主细胞核内、宿主细胞的消化细胞器以及细胞质中均有观察到。感染鸟疫病毒的细胞含有几种代表其生命周期各阶段的形态。由核糖体和DNA链组成的“初始小体”在形态上与立克次氏体相似。在感染人型支原体的培养物中，大多数细胞变得又大又多核。尽管从这些培养物中很容易培养出人型支原体，但在电子显微镜制剂中只能发现少数几个。这些病原体位于细胞外，缺乏细胞壁，仅由一层单位膜包裹。同样，其内部成分是核糖体和DNA链。在本文采用的统一制备条件下，这三类病原体在形态上彼此可区分。
