Vanrompay D, Charlier G, Ducatelle R, Haesebrouck F
Department of Avian Medicine and Pathology, Faculty of Veterinary Medicine, Merelbeke, Belgium.
Infect Immun. 1996 Apr;64(4):1265-71. doi: 10.1128/iai.64.4.1265-1271.1996.
In order to find an explanation for the observed differences in levels of pathogenicity in turkeys of Chlamydia psittaci 84/55 (avian serovar A), 89/1326 (avian serovar B), 92/1293 (avian serovar D), and the Texas Turkey strain (avian serovar D) (P.B. Wyrick, J. Choong, S.T. Knight, D. Goyeau, E.S. Stuart, and A.B. MacDonald, Immunol. Infect. Dis. 4:131-141, 1994), the reproductive cycles of organisms of the four strains were studied in Buffalo Green Monkey cells by transmission electron microscopy, immunoelectron microscopy, and flow cytometry. Organisms of strains most pathogenic in turkeys, namely, the serovar A strain and the 92/1293 serovar D strain, (i) replicated faster, since at 50 h postinoculation significantly larger inclusions with more numerous infectious organisms were observed than with the less pathogenic strains; (ii) were often found devoid of inclusion membranes scattered throughout the cytoplasms; and (iii) induced severe degenerative changes in Buffalo Green Monkey cells. By immunoelectron microscopy and flow cytometry, chlamydial antigens could not be detected in the plasma membranes of infected host cells. However, the presence of chlamydial antigens in inclusion membranes was demonstrated by immunoelectron microscopy.
为了解释观察到的鹦鹉热衣原体84/55株（禽血清型A）、89/1326株（禽血清型B）、92/1293株（禽血清型D）和得克萨斯火鸡株（禽血清型D）在火鸡中的致病性水平差异（P.B. 怀里克、J. 钟、S.T. 奈特、D. 戈约、E.S. 斯图尔特和A.B. 麦克唐纳，《免疫与感染疾病》4:131 - 141，1994年），通过透射电子显微镜、免疫电子显微镜和流式细胞术在布法罗绿猴细胞中研究了这四株菌株的繁殖周期。在火鸡中致病性最强的菌株，即血清型A菌株和92/1293血清型D菌株，（i）复制更快，因为在接种后50小时观察到，与致病性较弱的菌株相比，包涵体明显更大，感染性生物体数量更多；（ii）经常发现其没有包涵体膜，散布在整个细胞质中；（iii）在布法罗绿猴细胞中诱导严重的退行性变化。通过免疫电子显微镜和流式细胞术，在受感染宿主细胞的质膜中未检测到衣原体抗原。然而，免疫电子显微镜证明了包涵体膜中存在衣原体抗原。
