利用四硝基蓝四氮唑结合电子显微镜技术对枯草芽孢杆菌中琥珀酸脱氢酶系统进行的演示。
The demonstration of the succinic dehydrogenase system in Bacillus subtilis using tetranitro--blue tetrazolium combined with techniques of electron microscopy.
作者信息
Sedar A W, Burde R M
出版信息
J Cell Biol. 1965 Oct;27(1):53-66. doi: 10.1083/jcb.27.1.53.
Abstract
Activity of the succinic dehydrogenase system was studied in Bacillus subtilis utilizing combined techniques of cytochemistry and electron microscopy. Organisms were incubated in a medium containing tetranitro-blue tetrazolium (TNBT) which served as an electron acceptor. Enzymatic activity, as evidenced by deposition of TNBT-formazan, was found on membranous organelles associated with the cytoplasmic membrane and septal plasma membrane, the nuclear area, and the plasma membrane. Flagella, approximately 190 A in diameter, with thorn-like projections protruded through the cell wall. Tangential-oblique sections of the cell wall showed many pores approximately 220 A in diameter with a center-to-center spacing of approximately 450 A.
摘要
利用细胞化学和电子显微镜相结合的技术，对枯草芽孢杆菌中的琥珀酸脱氢酶系统活性进行了研究。将生物体在含有四硝基蓝四唑（TNBT）的培养基中培养，TNBT作为电子受体。通过TNBT-甲臜的沉积证明，酶活性存在于与细胞质膜和隔膜质膜、核区域以及质膜相关的膜性细胞器上。直径约190埃的鞭毛带有刺状突起，穿过细胞壁伸出。细胞壁的切向斜切面显示出许多直径约220埃的孔，中心间距约为450埃。
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