ROSA C G, TSOU K C
J Cell Biol. 1963 Mar;16(3):445-54. doi: 10.1083/jcb.16.3.445.
Succinic dehydrogenase (SDH) was localized in the mitochondria of Sarcoma 37 ascites tumor cells by the use of tetranitro-BT (TNBT) and nitro-BT (NBT) in smear preparations. Results with each tetrazolium salt as electron acceptor were evaluated with respect to: (a) size and shape of the formazan precipitate relative to standard mitochondrial morphology; (b) crystallization phenomena of reduced dye; (c) lipid adsorption of formazan. The association of formazan- or iron hematoxylin-stained mitochondria with lipid droplets within the cells was investigated, as was also the influence of formalin fixation, with and without cold acetone pretreatment, on mitochondrial morphology and enzymatic staining. Data from these studies appear to indicate that TNBT is more suitable than NBT for use as a cytochemical reagent in oxidative and/or dehydrogenase enzyme histochemistry and cytochemistry.
通过在涂片标本中使用四硝基蓝四氮唑（TNBT）和硝基蓝四氮唑（NBT），将琥珀酸脱氢酶（SDH）定位在肉瘤37腹水肿瘤细胞的线粒体中。以每种四氮唑盐作为电子受体的结果，根据以下方面进行评估：（a）相对于标准线粒体形态的甲臜沉淀的大小和形状；（b）还原染料的结晶现象；（c）甲臜的脂质吸附。研究了甲臜或铁苏木精染色的线粒体与细胞内脂质滴的关联，以及有无冷丙酮预处理的福尔马林固定对线粒体形态和酶染色的影响。这些研究的数据似乎表明，在氧化和/或脱氢酶组织化学和细胞化学中，TNBT比NBT更适合用作细胞化学试剂。
