Rutberg B, Hederstedt L, Holmgren E, Rutberg L
J Bacteriol. 1978 Oct;136(1):304-11. doi: 10.1128/jb.136.1.304-311.1978.
Eleven succinic dehydrogenase (SDH) mutants in Bacillus subtilis were analyzed by crossed immunoelectrophoresis with antiserum prepared against wild-type B. subtilis cytoplasmic membrane. A precipitate which stained for SDH was found in Triton X-100-solubilized wild-type membranes and in membranes from two of the SDH mutants. The remaining nine mutants did not show an SDH-staining precipitate. The respective mutations in these nine mutants all map in one locus, citF (Ohné et al., J. Bacteriol. 115:738-745, 1973). An SDH-specific antiserum was prepared by immunizing rabbits with the SDH precipitate obtained in crossed immunoelectrophoresis with solubilized wild-type membrane. Using this antiserum, it was shown that all of the nine citF mutants lack an SDH-specific antigen in the membrane but five of the citF mutants have a soluble SDH-specific antigen. No major differences were found in sodium dodecyl sulfatepolyacrylamide gels of membrane proteins from wild-type B. subtilis and from SDH mutants. A model for the organization of SDH in B. subtilis is proposed.
利用针对野生型枯草芽孢杆菌细胞质膜制备的抗血清，通过交叉免疫电泳分析了枯草芽孢杆菌中的11个琥珀酸脱氢酶（SDH）突变体。在经Triton X - 100溶解的野生型膜以及两个SDH突变体的膜中发现了一种可被SDH染色的沉淀。其余9个突变体未显示出SDH染色沉淀。这9个突变体中的相应突变均位于一个基因座citF（Ohné等人，《细菌学杂志》115:738 - 745，1973年）。用交叉免疫电泳中从溶解的野生型膜获得的SDH沉淀对兔子进行免疫，制备了一种SDH特异性抗血清。使用这种抗血清表明，所有9个citF突变体在膜中都缺乏SDH特异性抗原，但其中5个citF突变体有可溶性SDH特异性抗原。在野生型枯草芽孢杆菌和SDH突变体的膜蛋白十二烷基硫酸钠 - 聚丙烯酰胺凝胶中未发现重大差异。提出了枯草芽孢杆菌中SDH的组织模型。
