Taylor M J, Thorne C B
J Bacteriol. 1966 Jan;91(1):81-8. doi: 10.1128/jb.91.1.81-88.1966.
Taylor, Martha J. (Fort Detrick, Frederick, Md.), and Curtis B. Thorne. Concurrent changes in transducing efficiency and content of transforming deoxyribonucleic acid in Bacillus subtilis bacteriophage SP-10. J. Bacteriol. 91:81-88. 1966.-Spores of Bacillus subtilis W-23-S(r) infected with transducing phage SP-10 served as convenient inocula for broth cultures from which transducing phage was harvested. Methods are described for producing highly infected spores. The inoculum level of infected spores in nutrient broth-yeast extract-glucose medium affected the transducing efficiency of SP-10 in lysates of these cultures. Phage in lysates of cultures inoculated with about 10(5) or fewer spores per milliliter transduced 20- to 350-fold more efficiently than did phage in lysates from cultures inoculated with 10(6) to 10(7) spores per milliliter. Transduction frequencies in the order of 10(-5) per plaque-forming unit were obtained routinely, and some infected-spore preparations yielded phage that gave frequencies as high as 10(-4). The combination of inoculum level and incubation time required to produce the best transducing phage had to be determined empirically for each batch of infected spores. Several possible explanations for the difference between lysates having high (HTE) and those having low (LTE) transducing efficiency were ruled out by special experiments. The hypothesis is presented that some cultural condition resulting from a relatively low inoculum of phage-infected spores favors the incorporation by phage particles of bacterial deoxyribonucleic acid (DNA) in the manner required for the production of transducing phage. Support for this hypothesis is a demonstration, through transformation experiments with DNA extracted from HTE and LTE phage particles, that populations of HTE phage particles yielded significantly more (7 to 27 times) transforming activity per microgram of DNA than did populations of LTE phage.
泰勒，玛莎·J.（马里兰州弗雷德里克市迪特里克堡），以及柯蒂斯·B.索恩。枯草芽孢杆菌噬菌体SP - 10中转导效率与转化脱氧核糖核酸含量的同时变化。《细菌学杂志》91:81 - 88。1966年。——用转导噬菌体SP - 10感染的枯草芽孢杆菌W - 23 - S(r)的孢子作为肉汤培养物的便利接种物，从中收获转导噬菌体。描述了产生高度感染孢子的方法。营养肉汤 - 酵母提取物 - 葡萄糖培养基中感染孢子的接种水平影响了这些培养物裂解物中SP - 10的转导效率。每毫升接种约10⁵个或更少孢子的培养物裂解物中的噬菌体，其转导效率比每毫升接种10⁶至10⁷个孢子的培养物裂解物中的噬菌体高20至350倍。每噬菌斑形成单位的转导频率通常可达10⁻⁵量级，一些感染孢子制剂产生的噬菌体频率高达10⁻⁴。对于每一批感染孢子，都必须凭经验确定产生最佳转导噬菌体所需的接种水平和培养时间的组合。通过特殊实验排除了几种可能解释高转导效率（HTE）裂解物与低转导效率（LTE）裂解物之间差异的原因。提出的假设是，由相对低接种量的噬菌体感染孢子导致的某些培养条件有利于噬菌体颗粒以产生转导噬菌体所需的方式掺入细菌脱氧核糖核酸（DNA）。支持这一假设的是，通过对从HTE和LTE噬菌体颗粒中提取的DNA进行转化实验表明，每微克DNA，HTE噬菌体颗粒群体产生的转化活性比LTE噬菌体群体显著多（7至27倍）。
