Doebbler T K, Rose N R
Clin Exp Immunol. 1966 Apr;1(2):159-69.
Monkey antisera to monkey thyroid crude extract were studied by means of tanned cell haemagglutination, gel precipitation, and passive cutaneous anaphylaxis. They reacted with extracts of all monkey thyroids tested and not with extracts of other monkey organs. Cross-reactions occurred with thyroid extracts of several other mammalian species, especially with human thyroid antigen. Absorption and inhibition tests indicated the participation of a number of thyroid-specific antibodies, some of which were responsible for these cross-reactions, while other antibodies reacted only with homologous monkey thyroid. The antigen responsible for these reactions could be identified as thyroglobulin. The antibodies were resistant to treatment by sulphhydryl agents and heat, and sedimented as 7S globulins.
通过鞣酸细胞血凝反应、凝胶沉淀反应和被动皮肤过敏反应对猴抗猴甲状腺粗提取物血清进行了研究。它们与所有测试的猴甲状腺提取物发生反应，而不与其他猴器官的提取物发生反应。与其他几种哺乳动物的甲状腺提取物发生了交叉反应，尤其是与人甲状腺抗原发生交叉反应。吸收和抑制试验表明有多种甲状腺特异性抗体参与，其中一些抗体导致了这些交叉反应，而其他抗体仅与同源猴甲状腺发生反应。引起这些反应的抗原可鉴定为甲状腺球蛋白。这些抗体对巯基试剂和热处理具有抗性，并以7S球蛋白形式沉淀。
