Rose N R, Skelton F R, Kite J H, Witebsky E
Clin Exp Immunol. 1966 Apr;1(2):171-88.
Two rhesus monkeys were injected with monkey thyroid extract plus complete Freund adjuvant, and the immunopathological changes were followed for over a year. In one monkey the level of antibody to thyroglobulin, as measured by tanned cell haemagglutination, increased gradually over the period. Antibodies to constituents of the thyroid epithelial cells themselves were demonstrated by complement fixation and cytotoxic reactions. These titres were greatest between 80 and 150 days and then diminished. Skin test reactions of delayed hypersensitivity were present at the same time. As followed by repeated biopsies, the pathological changes progressed during the course of the disease from moderate infiltration to virtual replacement of all the thyroidal elements by fibrosis. The second monkey produced the highest titre of tanned cell haemagglutinating antibody early in immunization, followed by gradual decrease. Complement-fixing antibodies were present primarily between days 150 and 200, and then disappeared. Cytotoxic antibodies were insignificant in the samples tested and only minimal skin reactions were elicited. The first biopsy taken after 100 days showed severe thyroiditis. Subsequent examinations revealed regression and regeneration so that 18 months after initiation of the experiment, the thyroid gland was nearly normal in appearance.
给两只恒河猴注射猴甲状腺提取物加完全弗氏佐剂，并对其免疫病理变化进行了一年多的跟踪观察。在一只猴子中，通过鞣酸细胞血凝试验测定的抗甲状腺球蛋白抗体水平在这段时间内逐渐升高。通过补体结合和细胞毒性反应证明了针对甲状腺上皮细胞自身成分的抗体。这些滴度在80至150天之间最高，然后下降。同时出现了迟发型超敏反应的皮肤试验反应。通过反复活检观察到，疾病过程中病理变化从轻度浸润发展为所有甲状腺成分几乎被纤维组织替代。第二只猴子在免疫早期产生了最高滴度的鞣酸细胞血凝抗体，随后逐渐下降。补体结合抗体主要出现在150至200天之间，然后消失。在所检测的样本中，细胞毒性抗体不明显，仅引起轻微的皮肤反应。100天后进行的首次活检显示为重度甲状腺炎。随后的检查发现有消退和再生现象，因此在实验开始18个月后，甲状腺外观几乎正常。
