Wachsman J T, Hogg L
J Bacteriol. 1964 May;87(5):1137-9. doi: 10.1128/jb.87.5.1137-1139.1964.
Wachsman, J. T. (University of Illinois, Urbana), and L. Hogg. Use of 5-fluorouracil for the isolation of auxotrophic mutants of Bacillus megaterium. J. Bacteriol. 87:1137-1139. 1964.-The combination of 5-fluorouracil (FU) and uridine was used to selectively kill wild-type cells of Bacillus megaterium KM, thereby providing surviving populations greatly enriched in auxotrophic mutants. Exponentially growing cells were irradiated with ultraviolet light, incubated in a basal medium containing sucrose and, in most experiments, a complete amino acid mixture. Exponentially growing cells were then washed and incubated in the basal medium containing only sucrose, to deplete intracellular reserves. FU and uridine were added, and incubation was continued. After 5 hr, auxotrophs may account for up to 50% of the survivors. Organisms requiring each of the following compounds were identified: alanine, arginine, asparagine, cysteine, histidine, phenylalanine, serine, threonine, tyrosine, adenine, and guanine.
瓦克斯曼，J. T.（伊利诺伊大学厄巴纳分校）和L. 霍格。5-氟尿嘧啶用于巨大芽孢杆菌营养缺陷型突变体的分离。《细菌学杂志》87:1137 - 1139。1964年。——5-氟尿嘧啶（FU）和尿苷的组合被用于选择性杀死巨大芽孢杆菌KM的野生型细胞，从而使存活群体中营养缺陷型突变体大大富集。对数生长期的细胞用紫外线照射，在含有蔗糖以及在大多数实验中含有完整氨基酸混合物的基础培养基中培养。然后将对数生长期的细胞洗涤并在仅含蔗糖的基础培养基中培养，以耗尽细胞内储备。加入FU和尿苷，并继续培养。5小时后，营养缺陷型可能占存活者的50%。鉴定出了需要以下每种化合物的生物体：丙氨酸、精氨酸、天冬酰胺、半胱氨酸、组氨酸、苯丙氨酸、丝氨酸、苏氨酸、酪氨酸、腺嘌呤和鸟嘌呤。
