MANGALO R, WACHSMAN J T
J Bacteriol. 1962 Jan;83(1):27-34. doi: 10.1128/jb.83.1.27-34.1962.
Mangalo, R. (University of Illinois, Urbana) and J. T. Wachsman. Effect of 8-azaguanine on growth and viability of Bacillus megaterium. J. Bacteriol. 83:27-34. 1962.-The addition of 8-azaguanine to exponentially growing cells of Bacillus megaterium results in an inhibition of growth after a lag of approximately 30 min. However, 8-azaguanine-2-C(14) is incorporated into the nucleic acids in a linear fashion without a detectable lag. The inhibitory action is reversed by purines and their derivatives, but not by uridine, thymidine, or cytidine. 8-Azaguanine is bactericidal, especially under conditions where growth (ribonucleic acid synthesis) is possible. Growth in the presence of a complete amino acid mixture, either before or during exposure to 8-azaguanine, increases the rate of killing. Chloramphenicol has little or no effect on the bactericidal action of the analogue.
曼加洛，R.（伊利诺伊大学厄巴纳分校）和J.T.瓦克斯曼。8-氮杂鸟嘌呤对巨大芽孢杆菌生长和活力的影响。《细菌学杂志》83:27 - 34。1962年。——向指数生长期的巨大芽孢杆菌细胞中添加8-氮杂鸟嘌呤，约30分钟的延迟后会导致生长受到抑制。然而，8-氮杂鸟嘌呤-2-C（14）以线性方式掺入核酸中，没有可检测到的延迟。嘌呤及其衍生物可逆转这种抑制作用，但尿苷、胸苷或胞苷则不能。8-氮杂鸟嘌呤具有杀菌作用，尤其是在可能生长（核糖核酸合成）的条件下。在接触8-氮杂鸟嘌呤之前或期间，在完整氨基酸混合物存在下生长会增加杀灭速率。氯霉素对该类似物的杀菌作用几乎没有影响。
