抗生素U - 20,661的作用模式。
Mode of action of antibiotic U-20,661.
作者信息
Reusser F
出版信息
J Bacteriol. 1967 Jan;93(1):65-70. doi: 10.1128/jb.93.1.65-70.1967.
Abstract
Antibiotic U-20,661 was shown to inhibit predominantly deoxyribonucleic acid (DNA)-directed ribonucleic acid (RNA) synthesis by binding to the double-stranded DNA template. Specific binding to DNA was verified by difference spectroscopy, reversal of the RNA polymerase inhibitory effect by increasing concentrations of DNA template, and by moderately increasing the melting temperature of double-stranded DNA in the presence of the antibiotic. The RNA polymerase reaction primed with synthetic poly dAT was inhibited considerably, but not completely even with high concentrations of antibiotic. Thus, the agent might bind to adenine or thymidine or both bases in the double-stranded DNA helix.
摘要
抗生素U - 20,661被证明主要通过与双链DNA模板结合来抑制脱氧核糖核酸（DNA）指导的核糖核酸（RNA）合成。通过差示光谱法、增加DNA模板浓度可逆转RNA聚合酶抑制作用以及在抗生素存在下适度提高双链DNA的解链温度，证实了其与DNA的特异性结合。用合成的聚dAT引发的RNA聚合酶反应受到显著抑制，但即使使用高浓度抗生素也未完全抑制。因此，该药物可能与双链DNA螺旋中的腺嘌呤或胸腺嘧啶或两者结合。
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