Department of Microbial Chemistry, Graduate School of Pharmaceutical Sciences, Kitasato University, Tokyo 108-8641, Japan.
Medicinal Research Laboratories, School of Pharmacy, Kitasato University, Tokyo 108-8641, Japan.
Molecules. 2020 Oct 27;25(21):4971. doi: 10.3390/molecules25214971.
Among four mycobacteria, , , BCG and () , we established a silkworm infection assay with . When silkworms (fifth-instar larvae, = 5) were infected through the hemolymph with (7.5 × 10 CFU/larva) and bred at 37 °C, they all died around 40 h after injection. Under the conditions, clarithromycin and amikacin, clinically used antimicrobial agents, exhibited therapeutic effects in a dose-dependent manner. Furthermore, five kinds of microbial compounds, lariatin A, nosiheptide, ohmyungsamycins A and B, quinomycin and steffimycin, screened in an in vitro assay to observe anti- activity from 400 microbial products were evaluated in this silkworm infection assay. Lariatin A and nosiheptide exhibited therapeutic efficacy. The silkworm infection model with is useful to screen for therapeutically effective anti- antibiotics.
在四种分枝杆菌（ 、 、 、BCG 和 ）中，我们建立了一种使用 的家蚕感染模型。当家蚕（五龄幼虫， = 5）通过血腔感染 （7.5×10 CFU/幼虫）并在 37°C 下饲养时，它们在注射后约 40 小时全部死亡。在这些条件下，临床上使用的两种抗菌药物克拉霉素和阿米卡星以剂量依赖的方式表现出治疗效果。此外，从 400 种微生物产物中筛选出的五种微生物化合物拉瑞他汀 A、诺斯替肽、ohmyungsamycins A 和 B、醌霉素和 steffimycin 在体外测定中观察到抗 活性，在该家蚕感染模型中进行了评估。拉瑞他汀 A 和诺斯替肽表现出治疗效果。使用 的家蚕感染模型可用于筛选具有治疗效果的抗 抗生素。
