Shuster R C
J Bacteriol. 1967 Mar;93(3):811-5. doi: 10.1128/jb.93.3.811-815.1967.
The fate of ultraviolet-induced, thymine-containing dimers in the deoxyribonucleic acid (DNA) of Bacillus subtilis was investigated in both the wild type (UV(R)) and an ultraviolet light-sensitive (UV(S)) mutant. During incubation in the dark, dimers were excised from the DNA of the UV(R)B. subtilis, but remained in the DNA of the UV(S) mutant. About 40% of the excised dimers recovered in the wild type were in the acid-soluble fraction; the remainder were in the incubation medium. A UV(S) mutant of Escherichia coli K-12, shown previously to be defective in dimer excision, was irradiated with ultraviolet light and incubated under visible light for 3 hr. About 65% of thymine-containing photoproducts were removed from the DNA. These photoproducts were not recovered in the acid-soluble fraction. In comparison, the UV(S) mutant of B. subtilis lost only 13% of such photoproducts from DNA when exposed to light under the same conditions.
在野生型（UV(R)）和紫外线敏感型（UV(S)）突变体中，研究了紫外线诱导的枯草芽孢杆菌脱氧核糖核酸（DNA）中含胸腺嘧啶二聚体的命运。在黑暗中孵育期间，二聚体从UV(R)枯草芽孢杆菌的DNA中被切除，但仍留在UV(S)突变体的DNA中。在野生型中回收的约40%被切除的二聚体存在于酸溶性部分；其余的存在于孵育培养基中。先前已证明在二聚体切除方面有缺陷的大肠杆菌K-12的UV(S)突变体，用紫外线照射并在可见光下孵育3小时。约65%的含胸腺嘧啶光产物从DNA中被去除。这些光产物未在酸溶性部分中回收。相比之下，在相同条件下暴露于光时，枯草芽孢杆菌的UV(S)突变体仅从DNA中损失了13%的此类光产物。
